Hydroxypropyl methylcellulose (HPMC) film containing bovine bone collagen (BC) and nano-TiO2 were developed via casting method. The films were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR), and their mechanical properties, barrier properties, thermal stability and surface color were investigated. The results showed that polymer matrices of HPMC and BC had good compatibility. The nano-TiO2 could be well embedded and dispersed in the matrix of HPMC/BC films, resulting in homogeneous, continuous and compact structure of the composite films. The addition of BC and nano-TiO2 significantly (p < .05) changed the surface color parameters of the films (p < .05). In addition, the introduction of BC and nano-TiO2 had a positive effect on the improvement of mechanical properties, barrier properties and thermal stability of the HPMC based films. The results suggest that HPMC based films containing BC and nano-TiO2 are more suitable for active packaging in the food industry.