BIOMAT Database Logo BIOMAT Database Logo

Crystallographic Studies of Triosephosphate Isomerase from Schistosoma mansoni. 2020

Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.

Protein structure determination by X-ray crystallography guides structure-function and rational drug design studies. Helminths cause devastating diseases, including schistosomiasis that affects over one-third of the human population. Trematodes from the genus Schistosoma heavily depend on glycolysis; thus enzymes involved in this metabolic pathway are potential drug targets. Here we present a protocol to obtain crystal structures of recombinantly expressed triosephosphate isomerase from S. mansoni (SmTPI) that diffracted in house to a resolution of 2 Å.

UI MeSH Term Description Entries
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012550 Schistosoma mansoni A species of trematode blood flukes of the family Schistosomatidae. It is common in the Nile delta. The intermediate host is the planorbid snail. This parasite causes schistosomiasis mansoni and intestinal bilharziasis. Schistosoma mansonus,mansonus, Schistosoma
D014305 Triose-Phosphate Isomerase An enzyme that catalyzes reversibly the conversion of D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate. A deficiency in humans causes nonspherocytic hemolytic disease (ANEMIA, HEMOLYTIC, CONGENITAL NONSPHEROCYTIC). EC 5.3.1.1. Phosphotriose Isomerase,Triosephosphate Isomerase,Triosephosphate Mutase,Isomerase, Phosphotriose,Isomerase, Triose-Phosphate,Isomerase, Triosephosphate,Mutase, Triosephosphate,Triose Phosphate Isomerase
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies

Related Publications

Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Characterization of the Schistosoma mansoni gene encoding the glycolytic enzyme, triosephosphate isomerase.
June 1993, Molecular and biochemical parasitology,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Preliminary crystallographic studies of triosephosphate isomerase (TIM) from the hyperthermophilic Archaeon Pyrococcus woesei.
November 1998, Acta crystallographica. Section D, Biological crystallography,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Preliminary crystallographic studies of triosephosphate isomerase from the blood parasite Trypanosoma brucei brucei.
September 1984, Journal of molecular biology,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Studies of the phosphoglucose isomerase of Schistosoma mansoni.
August 1955, The Journal of biological chemistry,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Crystallographic binding studies with an engineered monomeric variant of triosephosphate isomerase.
August 2010, Acta crystallographica. Section D, Biological crystallography,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Bacterial expression and characterization of functional recombinant triosephosphate isomerase from Schistosoma japonicum.
December 1999, Protein expression and purification,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Triosephosphate isomerase from yeast.
January 1975, Methods in enzymology,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Human triosephosphate isomerase cDNA and protein structure. Studies of triosephosphate isomerase deficiency in man.
March 1985, The Journal of biological chemistry,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Triosephosphate isomerase from human erythrocytes.
January 1975, Methods in enzymology,
Pedro Jimenez-Sandoval, and Eduardo Castro-Torres, and Corina Diaz-Quezada, and Luis G Brieba
Triosephosphate isomerase from rabbit liver.
January 1975, Methods in enzymology,
Copied contents to your clipboard!