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A rapid, convenient assay for flavin-containing monooxygenase activity is described. The method is based on direct analysis of quenched incubation mixtures by reverse-phase liquid chromatography, and utilizes p-nitrophenyl-1,3-oxathiolane as the substrate. The synthesis of the substrate and the product are described. The usefulness of p-nitrophenyl-1,3-oxathiolane S-oxide formation as a measure of flavin-containing monooxygenase activity was demonstrated using highly purified and microsomal hog and rat liver flavin-containing monooxygenase. The assay is especially useful for determining stereoselectivity of flavin-containing monooxygenase activity in small amounts of crude tissue preparations.
J R Cashman, and
J Proudfoot
February 1987,
Analytical biochemistry,
J R Cashman, and
J Proudfoot
February 1980,
Analytical biochemistry,
J R Cashman, and
J Proudfoot
November 1993,
Analytical biochemistry,
J R Cashman, and
J Proudfoot
January 1981,
Biochemical and biophysical research communications,
J R Cashman, and
J Proudfoot
January 1986,
Advances in experimental medicine and biology,
J R Cashman, and
J Proudfoot
January 1987,
Drug metabolism and disposition: the biological fate of chemicals,
J R Cashman, and
J Proudfoot
October 2011,
Proceedings of the National Academy of Sciences of the United States of America,
J R Cashman, and
J Proudfoot
July 1996,
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie,
J R Cashman, and
J Proudfoot
May 2019,
Xenobiotica; the fate of foreign compounds in biological systems,
J R Cashman, and
J Proudfoot
May 1984,
Journal of neurochemistry,
