Surface modification and bioconjugation of anti-CD4 monoclonal antibody to magnetic nanoparticles as a highly efficient affinity adsorbent for positive selection of peripheral blood T CD4+ lymphocytes. 2020

Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Magnetic activated cell sorting (MACS) is a straightforward and time-saving procedure for isolation of different healthy functional cells. The present study aimed for the developing of a new MACS-based platform for isolation of peripheral blood T CD4+ lymphocytes. For this goal, first: Fe3O4 magnetic nanoparticles (MNP) were prepared by co-precipitation of Fe (III) and Fe (II) ions and then coated by SiO2 shell, followed by the grafting of N-(phosphonomethyl) iminodiacetic acid (PMIDA) on the surface of fabricated MNP, Fe3O4@SiO2@PMIDA were formed. These MNP were further tested for their ability to bind CD4 T lymphocytes. Through conjugation of the anti-CD4 monoclonal antibody on the surface of Fe3O4@SiO2@PMIDA MNP. The newly developed immunomagnetic particles efficiently isolated T CD4+ lymphocytes from whole blood with high purity Therefore, our MNP afford an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application.

UI MeSH Term Description Entries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D015496 CD4-Positive T-Lymphocytes A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes. T4 Cells,T4 Lymphocytes,CD4-Positive Lymphocytes,CD4 Positive T Lymphocytes,CD4-Positive Lymphocyte,CD4-Positive T-Lymphocyte,Lymphocyte, CD4-Positive,Lymphocytes, CD4-Positive,T-Lymphocyte, CD4-Positive,T-Lymphocytes, CD4-Positive,T4 Cell,T4 Lymphocyte
D015704 CD4 Antigens 55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. They are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. T4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120. Antigens, CD4,CD4 Molecule,CD4 Receptor,CD4 Receptors,Receptors, CD4,T4 Antigens, T-Cell,CD4 Antigen,Receptors, Surface CD4,Surface CD4 Receptor,Antigen, CD4,Antigens, T-Cell T4,CD4 Receptor, Surface,CD4 Receptors, Surface,Receptor, CD4,Surface CD4 Receptors,T-Cell T4 Antigens,T4 Antigens, T Cell
D058185 Magnetite Nanoparticles Synthesized magnetic particles under 100 nanometers used in many biomedical applications including DRUG DELIVERY SYSTEMS and CONTRAST AGENTS. The particles are usually coated with a variety of polymeric compounds. Magnetite SPIONs,Magnetite Superparamagnetic Iron Oxide Nanoparticles,Superparamagnetic Magnetite Nanoparticles,Magnetite Nanoparticle,Magnetite Nanoparticle, Superparamagnetic,Magnetite Nanoparticles, Superparamagnetic,Magnetite SPION,Nanoparticle, Magnetite,Nanoparticle, Superparamagnetic Magnetite,Nanoparticles, Magnetite,Nanoparticles, Superparamagnetic Magnetite,SPION, Magnetite,SPIONs, Magnetite,Superparamagnetic Magnetite Nanoparticle
D018189 Immunomagnetic Separation A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation. Immunomagnetic Bead Technique,Immunomagnetic Purging,Immunomagnetic Cell Separation,Bead Technique, Immunomagnetic,Bead Techniques, Immunomagnetic,Cell Separation, Immunomagnetic,Cell Separations, Immunomagnetic,Immunomagnetic Bead Techniques,Immunomagnetic Cell Separations,Immunomagnetic Purgings,Immunomagnetic Separations,Purging, Immunomagnetic,Purgings, Immunomagnetic,Separation, Immunomagnetic,Separation, Immunomagnetic Cell,Separations, Immunomagnetic,Separations, Immunomagnetic Cell

Related Publications

Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
March 1988, European journal of immunology,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
May 2014, Colloids and surfaces. B, Biointerfaces,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
July 2001, The European respiratory journal,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
July 1989, Journal of immunology (Baltimore, Md. : 1950),
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
May 1993, Molecular immunology,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
May 2017, Journal of immunological methods,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
December 2007, British journal of haematology,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
February 1997, European journal of immunology,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
November 2014, Cytometry. Part A : the journal of the International Society for Analytical Cytology,
Zahra Rashid, and Fazel Shokri, and Alireza Abbasi, and Mehdi Khoobi, and Amir-Hassan Zarnani
March 2010, Philosophical transactions. Series A, Mathematical, physical, and engineering sciences,
Copied contents to your clipboard!