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Human T lymphocytes express either alpha/beta- or gamma/delta-TCR in association with the CD3 complex. We have isolated a mAb, delta TCS1, that immunoprecipitated the gamma/delta-TCR heterodimer from cell lysates of Peer and Molt-13 leukemia cell lines. After dissociation of the gamma- and delta-chains of TCR by treatment with SDS, delta TCS1 specifically immunoprecipitated the delta-chain. This antibody bound to the surface of other gamma/delta-positive T cell lines and clones and was able to stimulate the proliferation of a minor cell population (0.9 to 4.0%) of resting human PBL. Upon binding to gamma/delta-TCR-bearing Molt-13 cells and PBL, delta TCS1 elicited a fura-2 Caa+ signal indicating that the gamma/delta-receptor is functionally similar to the alpha/beta-heterodimer. These data indicate that the delta TCS1 antibody recognizes an epitope on TCR delta-chain and its mitogenic activity should be useful in characterizing the functional properties of human gamma/delta-positive T lymphocytes.
Y J Wu, and
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C Rittershaus, and
P Rogers, and
L LaManna, and
S H Ip
April 2002,
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S H Ip
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S H Ip
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W T Tian, and
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C Rittershaus, and
P Rogers, and
L LaManna, and
S H Ip
September 1995,
Annals of the New York Academy of Sciences,
Y J Wu, and
W T Tian, and
R M Snider, and
C Rittershaus, and
P Rogers, and
L LaManna, and
S H Ip
January 1989,
Clinical immunology and immunopathology,
