Stem Cell Conditioned Medium Treatment for Canine Spinal Cord Injury: Pilot Feasibility Study. 2020

Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
Center of Experimental and Clinical Regenerative Medicine, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 04181 Kosice, Slovakia.

Spinal cord injury (SCI) involves nerve damage and often leads to motor, sensory and autonomic dysfunctions. In the present study, we have designed a clinical protocol to assess the feasibility of systemic delivery of allogenic canine bone marrow tissue-derived mesenchymal stem cell conditioned medium (BMMSC CM) to dogs with SCI. Four client-owned dogs with chronic SCI lasting more than six months underwent neurological and clinical evaluation, MRI imaging and blood tests before being enrolled in this study. All dogs received four intravenous infusions with canine allogenic BMMSC CM within one month. Between the infusions the dogs received comprehensive physiotherapy, which continued for three additional months. No adverse effects or complications were observed during the one, three and six months follow-up periods. Neither blood chemistry panel nor hematology profile showed any significant changes. All dogs were clinically improved as assessed using Olby locomotor scales after one, three and six months of BMMSC CM treatment. Furthermore, goniometric measurements revealed partial improvement in the range of joint motion. Bladder function improved in two disabled dogs. We conclude that multiple delivery of allogenic cell-derived conditioned medium to dogs with chronic SCI is feasible, and it might be clinically beneficial in combination with physiotherapy.

UI MeSH Term Description Entries
D008124 Locomotion Movement or the ability to move from one place or another. It can refer to humans, vertebrate or invertebrate animals, and microorganisms. Locomotor Activity,Activities, Locomotor,Activity, Locomotor,Locomotor Activities
D010865 Pilot Projects Small-scale tests of methods and procedures to be used on a larger scale if the pilot study demonstrates that these methods and procedures can work. Pilot Studies,Pilot Study,Pilot Project,Project, Pilot,Projects, Pilot,Studies, Pilot,Study, Pilot
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004283 Dog Diseases Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used. Canine Diseases,Canine Disease,Disease, Canine,Disease, Dog,Diseases, Canine,Diseases, Dog,Dog Disease
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D005240 Feasibility Studies Studies to determine the advantages or disadvantages, practicability, or capability of accomplishing a projected plan, study, or project. Feasibility Study,Studies, Feasibility,Study, Feasibility
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013119 Spinal Cord Injuries Penetrating and non-penetrating injuries to the spinal cord resulting from traumatic external forces (e.g., WOUNDS, GUNSHOT; WHIPLASH INJURIES; etc.). Myelopathy, Traumatic,Injuries, Spinal Cord,Post-Traumatic Myelopathy,Spinal Cord Contusion,Spinal Cord Laceration,Spinal Cord Transection,Spinal Cord Trauma,Contusion, Spinal Cord,Contusions, Spinal Cord,Cord Contusion, Spinal,Cord Contusions, Spinal,Cord Injuries, Spinal,Cord Injury, Spinal,Cord Laceration, Spinal,Cord Lacerations, Spinal,Cord Transection, Spinal,Cord Transections, Spinal,Cord Trauma, Spinal,Cord Traumas, Spinal,Injury, Spinal Cord,Laceration, Spinal Cord,Lacerations, Spinal Cord,Myelopathies, Post-Traumatic,Myelopathies, Traumatic,Myelopathy, Post-Traumatic,Post Traumatic Myelopathy,Post-Traumatic Myelopathies,Spinal Cord Contusions,Spinal Cord Injury,Spinal Cord Lacerations,Spinal Cord Transections,Spinal Cord Traumas,Transection, Spinal Cord,Transections, Spinal Cord,Trauma, Spinal Cord,Traumas, Spinal Cord,Traumatic Myelopathies,Traumatic Myelopathy
D017077 Culture Media, Conditioned Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis). Conditioned Culture Media,Conditioned Culture Medium,Conditioned Media,Conditioned Medium,Culture Medium, Conditioned,Media, Conditioned,Medium, Conditioned
D059630 Mesenchymal Stem Cells Mesenchymal stem cells, also referred to as multipotent stromal cells or mesenchymal stromal cells are multipotent, non-hematopoietic adult stem cells that are present in multiple tissues, including BONE MARROW; ADIPOSE TISSUE; and WHARTON JELLY. Mesenchymal stem cells can differentiate into mesodermal lineages, such as adipocytic, osteocytic and chondrocytic. Adipose Tissue-Derived Mesenchymal Stem Cell,Adipose Tissue-Derived Mesenchymal Stromal Cell,Adipose-Derived Mesenchymal Stem Cell,Bone Marrow Mesenchymal Stem Cell,Mesenchymal Stromal Cell,Mesenchymal Stromal Cells,Multipotent Bone Marrow Stromal Cell,Multipotent Mesenchymal Stromal Cell,Adipose Tissue-Derived Mesenchymal Stem Cells,Adipose Tissue-Derived Mesenchymal Stromal Cells,Adipose-Derived Mesenchymal Stem Cells,Adipose-Derived Mesenchymal Stromal Cells,Bone Marrow Mesenchymal Stem Cells,Bone Marrow Stromal Cell,Bone Marrow Stromal Cells,Bone Marrow Stromal Cells, Multipotent,Bone Marrow Stromal Stem Cells,Mesenchymal Progenitor Cell,Mesenchymal Progenitor Cells,Mesenchymal Stem Cell,Mesenchymal Stem Cells, Adipose-Derived,Mesenchymal Stromal Cells, Multipotent,Multipotent Bone Marrow Stromal Cells,Multipotent Mesenchymal Stromal Cells,Stem Cells, Mesenchymal,Wharton Jelly Cells,Wharton's Jelly Cells,Adipose Derived Mesenchymal Stem Cell,Adipose Derived Mesenchymal Stem Cells,Adipose Derived Mesenchymal Stromal Cells,Adipose Tissue Derived Mesenchymal Stem Cell,Adipose Tissue Derived Mesenchymal Stem Cells,Adipose Tissue Derived Mesenchymal Stromal Cell,Adipose Tissue Derived Mesenchymal Stromal Cells,Mesenchymal Stem Cells, Adipose Derived,Progenitor Cell, Mesenchymal,Progenitor Cells, Mesenchymal,Stem Cell, Mesenchymal,Stromal Cell, Mesenchymal,Stromal Cells, Mesenchymal,Wharton's Jelly Cell,Whartons Jelly Cells

Related Publications

Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
March 2017, Cell transplantation,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
January 2022, Frontiers in veterinary science,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
December 2018, Journal of clinical medicine,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
September 2003, The Veterinary record,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
January 2021, Journal of orthopaedic surgery (Hong Kong),
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
February 2024, Current stem cell research & therapy,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
September 2022, Spine surgery and related research,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
June 2020, International journal of molecular sciences,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
July 2010, Nature reviews. Neurology,
Zuzana Vikartovska, and Maria Kuricova, and Jana Farbakova, and Tomas Liptak, and Dagmar Mudronova, and Filip Humenik, and Aladar Madari, and Marcela Maloveska, and Eva Sykova, and Dasa Cizkova
January 2021, Cell transplantation,
Copied contents to your clipboard!