OBJECTIVE To compare 2 swept-source optical coherence tomography (SS-OCT) biometers, IOLMaster 700 (biometer A), and ANTERION (biometer B). METHODS Department of Ophthalmology, Vienna Institute for Research in Ocular Surgery, Karl Landsteiner Institute, Hanusch Hospital, Vienna, Austria. METHODS Retrospective study. METHODS Biometric measurements of cataract patients performed between June and July 2019 were reviewed. Patients scheduled for cataract surgery were measured with both SS-OCT devices on the same day. The following biometry parameters were compared: keratometry, central corneal thickness, anterior chamber depth (ACD), lens thickness (LT), and axial length (AL). RESULTS In total, 389 eyes of 209 subjects were measured with both devices. The mean absolute difference between the keratometry data of the 2 devices was 0.04 ± 0.05 mm (7.80 ± 0.26 mm for biometer A and 7.82 ± 0.26 mm for biometer B; P < .0001) for the steep keratometry readings and 0.04 ± 0.04 mm (7.63 ± 0.26 mm and 7.65 ± 0.25 mm; P < .0001) for the flat keratometry readings. For ACD and LT, the mean absolute difference was 0.07 ± 0.04 mm and 0.07 ± 0.04 mm. The mean absolute difference for AL was 0.02 ± 0.03 mm (23.55 ± 1.18 mm for biometer A and 23.54 ± 1.18 mm for biometer B; P < .0001). CONCLUSIONS Good agreement was found between the 2 devices, with a minor offset for ACD and LT measurements. Although differences were found to be small, the devices should not be used interchangeably.