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Three mouse monoclonal antibodies (mAb) directed against rat B lineage antigens were produced. The mAb, designated HIS14 (IgG1), HIS22 (IgM) and HIS24 (IgG2b), were characterized for binding to lymphoid and nonlymphoid tissues by immunoperoxidase staining of frozen sections and by (double-) immunofluorescence staining of single cell suspensions from lymphoid organs. HIS14 recognized a pan B cell determinant: it reacted with virtually all cells of each anatomic B cell compartment and with about 95% of surface (s)Ig+ cells in thoracic duct lymph and in suspensions of spleen and lymph nodes. HIS22 and HIS24 detected B lineage-associated antigens expressed by major subpopulations of B cells. HIS22 predominantly stained the lymphocyte corona, but not (or weakly) the germinal centers and splenic marginal zones, whereas HIS24 reacted with both corona and germinal center and not (or weakly) with marginal zone. In accordance with this, substantial proportions of sIg+ cells in spleen cell suspensions did not express HIS22 or HIS24 determinants (20% and 27%, respectively). In bone marrow the vast majority of cytomplasmic mu+ pre-B cells were HIS14+ and HIS24+, and up to one third also HIS22+, indicating an appearance of the determinants early in B lymphocytopoiesis. The antigens recognized by HIS14, HIS22 and HIS24 are lost during the final stage of B cell differentiation: none of the mAb bound to plasma cells. As far as detectable, neither cells of myeloid and erythroid lineages in bone marrow nor thymocytes were stained by HIS14, HIS22, or HIS24. In suspensions of peripheral lymphoid organs (spleen and lymph nodes) but not in thoracic duct lymph, HIS14 and HIS24 labeled a small proportion (12% and 14%, respectively) of Ig- cells. HIS22 did not bind to Ig- peripheral lymphocytes. Reactivity of HIS14, HIS22 and HIS24 with nonlymphoid tissues was virtually absent; HIS22 stained the high endothelial venules in lymph nodes and Peyer's patches. As determined by immunoblotting, the antigenic determinants on lymph node cells recognized by HIS14, HIS22 and HIS24 were present on molecules with an apparent molecular mass of 205 kDa, 210 (and 175) kDa and 205 kDa, respectively, which is similar to the molecular mass of the B cell form of the rat leukocyte common antigen. In addition, the antigens recognized by HIS14, HIS22 and HIS24 co-capped with the leukocyte common antigen. This suggests that each of the three mAb recognize determinants present on the B cell form of the leukocyte common antigen.
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
October 1983,
Journal of immunological methods,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
February 1990,
Nihon juigaku zasshi. The Japanese journal of veterinary science,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
April 1988,
Veterinary immunology and immunopathology,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
May 1985,
Journal of immunological methods,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
February 1989,
Proceedings of the National Academy of Sciences of the United States of America,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
December 1989,
The Tohoku journal of experimental medicine,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
September 1985,
Cancer research,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
December 1981,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
August 1988,
Immunology,
F G Kroese, and
A S Wubbena, and
D Opstelten, and
G J Deenen, and
E H Schwander, and
L De Leij, and
H Vos, and
S Poppema, and
J Volberda, and
P Nieuwenhuis
August 1977,
Israel journal of medical sciences,
