Calcium signaling in endocardial and epicardial ventricular myocytes from streptozotocin-induced diabetic rats. 2021

Lina T Al Kury, and Vadym Sydorenko, and Manal Ma Smail, and Muhammad A Qureshi, and Anatoly Shmygol, and Dimitrios Papandreou, and Jaipaul Singh, and Frank Christopher Howarth
Department of Health Sciences, College of Natural and Health Sciences, Zayed University, Abu Dhabi, United Arab Emirates.

OBJECTIVE Abnormalities in Ca2+ signaling have a key role in hemodynamic dysfunction in diabetic heart. The purpose of this study was to explore the effects of streptozotocin (STZ)-induced diabetes on Ca2+ signaling in epicardial (EPI) and endocardial (ENDO) cells of the left ventricle after 5-6 months of STZ injection. METHODS Whole-cell patch clamp was used to measure the L-type Ca2+ channel (LTCC) and Na+ /Ca2+ exchanger currents. Fluorescence photometry techniques were used to measure intracellular free Ca2+ concentration. RESULTS Although the LTCC current was not significantly altered, the amplitude of Ca2+ transients increased significantly in EPI-STZ and ENDO-STZ compared with controls. Time to peak LTCC current, time to peak Ca2+ transient, time to half decay of LTCC current and time to half decay of Ca2+ transients were not significantly changed in EPI-STZ and ENDO-STZ myocytes compared with controls. The Na+ /Ca2+ exchanger current was significantly smaller in EPI-STZ and in ENDO-STZ compared with controls. CONCLUSIONS STZ-induced diabetes resulted in an increase in amplitude of Ca2+ transients in EPI and ENDO myocytes that was independent of the LTCC current. Such an effect can be attributed, at least in part, to the dysfunction of the Na+ /Ca2+ exchanger. Additional studies are warranted to improve our understanding of the regional impact of diabetes on Ca2+ signaling, which will facilitate the discovery of new targeted treatments for diabetic cardiomyopathy.

UI MeSH Term Description Entries
D008297 Male Males
D003921 Diabetes Mellitus, Experimental Diabetes mellitus induced experimentally by administration of various diabetogenic agents or by PANCREATECTOMY. Alloxan Diabetes,Streptozocin Diabetes,Streptozotocin Diabetes,Experimental Diabetes Mellitus,Diabete, Streptozocin,Diabetes, Alloxan,Diabetes, Streptozocin,Diabetes, Streptozotocin,Streptozocin Diabete
D006352 Heart Ventricles The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation. Cardiac Ventricle,Cardiac Ventricles,Heart Ventricle,Left Ventricle,Right Ventricle,Left Ventricles,Right Ventricles,Ventricle, Cardiac,Ventricle, Heart,Ventricle, Left,Ventricle, Right,Ventricles, Cardiac,Ventricles, Heart,Ventricles, Left,Ventricles, Right
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013311 Streptozocin An antibiotic that is produced by Stretomyces achromogenes. It is used as an antineoplastic agent and to induce diabetes in experimental animals. Streptozotocin,2-Deoxy-2-((methylnitrosoamino)carbonyl)amino-D-glucose,Streptozotocine,Zanosar
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D019831 Sodium-Calcium Exchanger An electrogenic ion exchange protein that maintains a steady level of calcium by removing an amount of calcium equal to that which enters the cells. It is widely distributed in most excitable membranes, including the brain and heart. Ca(2+)-Na(+) Exchanger,Calcium-Sodium Carrier,Calcium-Sodium Exchanger,Na(+)-Ca(2+) Exchanger,Sodium-Calcium Carrier,Ca(2+)-Na(+) Antiporter,Calcium-Sodium Antiporter,Na(+)-Ca(2+) Antiporter,Sodium-Calcium Antiporter,Antiporter, Calcium-Sodium,Antiporter, Sodium-Calcium,Calcium Sodium Antiporter,Calcium Sodium Carrier,Calcium Sodium Exchanger,Carrier, Calcium-Sodium,Carrier, Sodium-Calcium,Exchanger, Calcium-Sodium,Exchanger, Sodium-Calcium,Sodium Calcium Antiporter,Sodium Calcium Carrier,Sodium Calcium Exchanger
D020013 Calcium Signaling Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins. Calcium Oscillations,Calcium Waves,Calcium Puffs,Calcium Sparks,Calcium Spikes,Calcium Oscillation,Calcium Puff,Calcium Signalings,Calcium Spark,Calcium Spike,Calcium Wave,Oscillation, Calcium,Oscillations, Calcium,Puff, Calcium,Puffs, Calcium,Signaling, Calcium,Signalings, Calcium,Spark, Calcium,Sparks, Calcium,Spike, Calcium,Spikes, Calcium,Wave, Calcium,Waves, Calcium
D020746 Calcium Channels, L-Type Long-lasting voltage-gated CALCIUM CHANNELS found in both excitable and non-excitable tissue. They are responsible for normal myocardial and vascular smooth muscle contractility. Five subunits (alpha-1, alpha-2, beta, gamma, and delta) make up the L-type channel. The alpha-1 subunit is the binding site for calcium-based antagonists. Dihydropyridine-based calcium antagonists are used as markers for these binding sites. Dihydropyridine Receptors,L-Type Calcium Channels,L-Type VDCC alpha-1 Subunit,L-Type Voltage-Dependent Calcium Channel,Long-Lasting Calcium Channel,Long-Lasting Calcium Channels,Receptors, Dihydropyridine,Dihydropyridine Receptor,L-Type Calcium Channel,L-Type VDCC,L-Type VDCC alpha-2 Subunit,L-Type VDCC beta Subunit,L-Type VDCC delta Subunit,L-Type VDCC gamma Subunit,L-Type Voltage-Dependent Calcium Channels,Calcium Channel, L-Type,Calcium Channel, Long-Lasting,Calcium Channels, L Type,Calcium Channels, Long-Lasting,Channel, Long-Lasting Calcium,L Type Calcium Channel,L Type Calcium Channels,L Type VDCC,L Type VDCC alpha 1 Subunit,L Type VDCC alpha 2 Subunit,L Type VDCC beta Subunit,L Type VDCC delta Subunit,L Type VDCC gamma Subunit,L Type Voltage Dependent Calcium Channel,L Type Voltage Dependent Calcium Channels,Long Lasting Calcium Channel,Long Lasting Calcium Channels,Receptor, Dihydropyridine,VDCC, L-Type

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