Biomaterial Database

RANKL-Based Osteoclastogenic Assay from Murine Bone Marrow Cells. 2021

Zhenqiang Yao, and Lianping Xing, and Brendan F Boyce

Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA.

The osteoclast is the unique type of cell that resorbs bone in vivo and it is required for normal skeletal development and postnatal homeostasis. Osteoclast deficiency impairs skeletal development during embryogenesis and results in osteopetrosis and impaired tooth eruption. In contrast, excessive osteoclast formation in adults results in bone loss in a number of conditions, including osteoporosis, rheumatoid arthritis, and metastatic bone disease. Osteoclasts are derived from monocytes/macrophages; they can be generated in vitro by treatment of these precursor cells with macrophage colony stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL). This chapter describes procedures for generating osteoclasts from mouse bone marrow cells in vitro using M-CSF and RANKL and assessing their ability to form resorption lacunae on thin bone slices.

UI	MeSH Term	Description	Entries
D010010	Osteoclasts	A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.	Odontoclasts,Cementoclast,Cementoclasts,Odontoclast,Osteoclast
D010012	Osteogenesis	The process of bone formation. Histogenesis of bone including ossification.	Bone Formation,Ossification, Physiologic,Endochondral Ossification,Ossification,Ossification, Physiological,Osteoclastogenesis,Physiologic Ossification,Endochondral Ossifications,Ossification, Endochondral,Ossifications,Ossifications, Endochondral,Osteoclastogeneses,Physiological Ossification
D001854	Bone Marrow Cells	Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.	Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016173	Macrophage Colony-Stimulating Factor	A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. M-CSF is a disulfide-bonded glycoprotein dimer with a MW of 70 kDa. It binds to a specific high affinity receptor (RECEPTOR, MACROPHAGE COLONY-STIMULATING FACTOR).	CSF-1,CSF-M,Colony-Stimulating Factor 1,Colony-Stimulating Factor, Macrophage,M-CSF,Colony Stimulating Factor 1,Colony Stimulating Factor, Macrophage
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D053245	RANK Ligand	A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. It plays an important role in regulating OSTEOCLAST differentiation and activation.	Tumor Necrosis Factor Ligand Superfamily Member 11,CD254 Antigen,OPGL Protein,Osteoclast Differentiation Factor,Osteoprotegerin Ligand,RANKL Protein,Receptor Activator of Nuclear Factor-kappa B Ligand,Receptor Activator of Nuclear Factor-kappaB Ligand,TNF Superfamily, Member 11,TRANCE Protein,Tumor Necrosis Factor-Related Activation-Induced Cytokine,Antigen, CD254,Differentiation Factor, Osteoclast,Receptor Activator of Nuclear Factor kappa B Ligand,Receptor Activator of Nuclear Factor kappaB Ligand,Tumor Necrosis Factor Related Activation Induced Cytokine
D018929	Cell Culture Techniques	Methods for maintaining or growing CELLS in vitro.	Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell