BIOMAT Database Logo BIOMAT Database Logo

DNase I- and micrococcal nuclease-hypersensitive sites in the human apolipoprotein B gene are tissue specific. 1988

B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140-0608.

We have mapped the DNase I- and micrococcal nuclease-hypersensitive sites present in the 5' end of the human apolipoprotein B (apo-B) gene in nuclei from cells expressing or not expressing the gene. Four DNase I-hypersensitive sites were found in nuclei from liver-derived HepG2 cells and intestine-derived CaCo-2 cells, which express the apo-B gene, but not in HeLa cells, which do not. These sites are located near positions -120, -440, -700, and +760 base pairs relative to the transcriptional start site. Undifferentiated CaCo-2 cells exhibited another site, near position -540. Six micrococcal nuclease-hypersensitive sites were found in nuclei from HepG2 and CaCo-2 cells, but not in HeLa cells or free DNA. These sites are located near positions -120, -390, -530, -700, -850, and +210. HepG2 cells exhibited another site, near position +460. Comparison of the DNA sequence of the 5' flanking regions of the human and mouse apo-B genes revealed a high degree of evolutionary conservation of short stretches of sequences in the immediate vicinity of each of the DNase I- and most of the micrococcal nuclease-hypersensitive sites.

UI MeSH Term Description Entries
D008836 Micrococcal Nuclease An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1. Staphylococcal Nuclease,TNase,Thermonuclease,Thermostable Nuclease,Nuclease, Micrococcal,Nuclease, Staphylococcal,Nuclease, Thermostable
D009707 Nucleosomes The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. This core is composed of the histones H2A, H2B, H3, and H4. Dinucleosomes,Polynucleosomes,Dinucleosome,Nucleosome,Polynucleosome
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D012045 Regulatory Sequences, Nucleic Acid Nucleic acid sequences involved in regulating the expression of genes. Nucleic Acid Regulatory Sequences,Regulatory Regions, Nucleic Acid (Genetics),Region, Regulatory,Regions, Regulatory,Regulator Regions, Nucleic Acid,Regulatory Region,Regulatory Regions
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002843 Chromatin The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. Chromatins
D003850 Deoxyribonuclease I An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA. DNase I,Streptodornase,DNA Endonuclease,DNA Nicking Enzyme,DNAase I,Dornavac,Endonuclease I,Nickase,Pancreatic DNase,T4-Endonuclease II,T7-Endonuclease I,Thymonuclease,DNase, Pancreatic,Endonuclease, DNA,T4 Endonuclease II,T7 Endonuclease I
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001055 Apolipoproteins B Major structural proteins of triacylglycerol-rich LIPOPROTEINS. There are two forms, apolipoprotein B-100 and apolipoprotein B-48, both derived from a single gene. ApoB-100 expressed in the liver is found in low-density lipoproteins (LIPOPROTEINS, LDL; LIPOPROTEINS, VLDL). ApoB-48 expressed in the intestine is found in CHYLOMICRONS. They are important in the biosynthesis, transport, and metabolism of triacylglycerol-rich lipoproteins. Plasma Apo-B levels are high in atherosclerotic patients but non-detectable in ABETALIPOPROTEINEMIA. Apo-B,Apo B,ApoB,Apoprotein (B),Apoproteins B

Related Publications

B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Tissue-specific DNase I hypersensitive sites in a foreign globin gene in transgenic mice.
December 1986, Nucleic acids research,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Digestion of insect chromatin with micrococcal nuclease, DNase I and DNase I combined with single-strand specific nuclease S1.
July 1977, Nucleic acids research,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
DNase I-hypersensitive sites are associated, in a tissue-specific manner, with expression of the calbindin-D9k-encoding gene.
December 1991, Gene,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
DNase I and micrococcal nuclease analysis of the tomato proteinase inhibitor I gene in chromatin.
January 1993, The Journal of biological chemistry,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Identification of tissue-specific DNase I hypersensitive sites in the rabbit flavin-containing monooxygenase form 2 gene.
August 1996, Drug metabolism and disposition: the biological fate of chemicals,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Mapping DNase-I-hypersensitive Sites.
June 2006, CSH protocols,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Tissue-specific DNase I-hypersensitive sites and hypomethylation in the chicken cardiac myosin light chain gene (L2-A).
October 1987, The Journal of biological chemistry,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Mapping DNase-I hypersensitive sites on human isochores.
August 2008, Gene,
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
In vivo analysis of DNase I hypersensitive sites in the human CFTR gene.
April 1999, Molecular medicine (Cambridge, Mass.),
B Levy-Wilson, and C Fortier, and B D Blackhart, and B J McCarthy
Nuclease-hypersensitive sites define a region with enhancer activity in the third intron of the human apolipoprotein B gene.
September 1992, The Journal of biological chemistry,
Copied contents to your clipboard!