Evaluation of 3 refractometers to determine transfer of passive immunity in neonatal beef calves. 2021

Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
Department of Production Animal Health, University of Calgary Faculty of Veterinary Medicine, Calgary, Alberta, Canada.

BACKGROUND Refractometry is used to assess transfer of passive immunity (TPI), but studies evaluating different refractometers and appropriate thresholds for recommended target immunoglobulin G (IgG) concentrations for beef calves are limited. OBJECTIVE To evaluate test performance of digital (DSTP) and optical (OSTP) serum total protein (STP) refractometers and a digital Brix (DBRIX) refractometer for assessment of passive immunity in beef calves. METHODS A total of 398 beef calves from 6 herds, 1 to 7 days of age. METHODS Serum IgG concentration was estimated by DSTP, OSTP, and DBRIX, and measured by radial immunodiffusion (RID). Correlation coefficients (r) among results were calculated. Optimal STP and Brix thresholds for identification of IgG <10, <16, and <24 g/L were determined using interval likelihood ratios. Refractometer performance and agreement were assessed using areas under the curve (AUC), diagnostic test characteristics, Cohen's kappa (κ), and Bland-Altman analysis. RESULTS Refractometer results were highly correlated with RID (r = 0.82-0.91) and with each other (r = 0.91-0.95), and overall test performance was excellent (AUC = 0.93-0.99). The STP concentrations of ≤5.1, ≤5.1, and ≤5.7 g/dL and Brix percentages of ≤7.9%, ≤8.3%, and ≤8.7% indicated IgG concentrations <10, <16, and <24 g/L, respectively. Agreement of refractometers with RID was variable (κ = 0.46-0.80) and among refractometers was substantial (κ = 0.62-0.89). CONCLUSIONS All refractometers showed good utility as monitoring tools for assessment of TPI in beef calves.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D012031 Refractometry Measurement of the index of refraction (the ratio of the velocity of light or other radiation in the first of two media to its velocity in the second as it passes from one into the other). Refractive Index,Index, Refractive,Indices, Refractive,Refractive Indices
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003955 Diagnostic Tests, Routine Diagnostic procedures, such as laboratory tests and x-rays, routinely performed on all individuals or specified categories of individuals in a specified situation, e.g., patients being admitted to the hospital. These include routine tests administered to neonates. Admission Tests, Routine,Hospital Admission Tests,Physical Examination, Preadmission,Routine Diagnostic Tests,Admission Tests, Hospital,Diagnostic Test, Routine,Diagnostic Tests,Examination, Preadmission Physical,Preadmission Physical Examination,Routine Diagnostic Test,Test, Routine Diagnostic,Tests, Diagnostic,Tests, Hospital Admission,Tests, Routine Diagnostic,Admission Test, Hospital,Admission Test, Routine,Diagnostic Test,Examinations, Preadmission Physical,Hospital Admission Test,Physical Examinations, Preadmission,Preadmission Physical Examinations,Routine Admission Test,Routine Admission Tests,Test, Diagnostic,Test, Hospital Admission,Test, Routine Admission,Tests, Routine Admission
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D019540 Area Under Curve A statistical means of summarizing information from a series of measurements on one individual. It is frequently used in clinical pharmacology where the AUC from serum levels can be interpreted as the total uptake of whatever has been administered. As a plot of the concentration of a drug against time, after a single dose of medicine, producing a standard shape curve, it is a means of comparing the bioavailability of the same drug made by different companies. (From Winslade, Dictionary of Clinical Research, 1992) AUC,Area Under Curves,Curve, Area Under,Curves, Area Under,Under Curve, Area,Under Curves, Area

Related Publications

Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
January 2011, Journal of veterinary internal medicine,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
December 2022, Preventive veterinary medicine,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
January 2015, Journal of veterinary internal medicine,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
April 2019, Animal : an international journal of animal bioscience,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
October 2017, Journal of dairy science,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
March 2023, Veterinary clinical pathology,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
August 2023, Veterinary sciences,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
July 2019, Equine veterinary journal,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
October 2022, Preventive veterinary medicine,
Lisa Gamsjäger, and Ibrahim Elsohaby, and Jennifer M Pearson, and Michel Levy, and Edmond A Pajor, and M Claire Windeyer
June 2020, Journal of dairy science,
Copied contents to your clipboard!