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Zinc uptake was studied in synaptosomes, isolated by the Ficoll flotation technique, using the radiotracer 65Zn. True uptake of zinc could be discriminated from binding to the outside of the synaptosomes by the absence of accumulation at 0 degree C and the dependency of the rate of uptake on the medium osmolarity. The zinc uptake, studied in the presence of various zinc-complexing agents, showed saturation kinetics when analyzed in terms of [Zn]free, yielding Km = 0.25 microM. The zinc uptake was independent of both ATP and the Na+ gradient. No efflux of zinc could be demonstrated from preloaded synaptosomes due to the formation of insoluble zinc complexes inside the synaptosomes. The results are discussed in terms of the modulation of diverse neurochemical processes by zinc.
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
January 1979,
Zeitschrift fur Naturforschung. Section C, Biosciences,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
August 1980,
Brain research,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
March 1990,
Biochemical pharmacology,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
December 1968,
The Biochemical journal,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
January 1982,
Toxicology,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
January 1987,
Journal of neural transmission,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
October 1994,
Anesthesiology,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
August 2006,
European journal of pharmacology,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
June 1996,
The Journal of pharmacology and experimental therapeutics,
J Wensink, and
A J Molenaar, and
U D Woroniecka, and
C J Van den Hamer
March 1994,
The Journal of nutrition,
