The new kid on the block: a dominant-negative mutation of phototropin1 enhances carotenoid content in tomato fruits. 2021

Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India.

Phototropins, the UVA-blue light photoreceptors, endow plants to detect the direction of light and optimize photosynthesis by regulating positioning of chloroplasts and stomatal gas exchange. Little is known about their functions in other developmental responses. A tomato Non-phototropic seedling1 (Nps1) mutant, bearing an Arg495His substitution in the vicinity of LOV2 domain in phototropin1, dominant-negatively blocks phototropin1 responses. The fruits of Nps1 mutant were enriched in carotenoids, particularly lycopene, compared with its parent, Ailsa Craig. On the contrary, CRISPR/CAS9-edited loss of function phototropin1 mutants displayed subdued carotenoids compared with the parent. The enrichment of carotenoids in Nps1 fruits is genetically linked with the mutation and exerted in a dominant-negative fashion. Nps1 also altered volatile profiles with high levels of lycopene-derived 6-methyl 5-hepten2-one. The transcript levels of several MEP and carotenogenesis pathway genes were upregulated in Nps1. Nps1 fruits showed altered hormonal profiles with subdued ethylene emission and reduced respiration. Proteome profiles showed a causal link between higher carotenogenesis and increased levels of protein protection machinery, which may stabilize proteins contributing to MEP and carotenogenesis pathways. The enhancement of carotenoid content by Nps1 in a dominant-negative fashion offers a potential tool for high lycopene-bearing hybrid tomatoes.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D002338 Carotenoids The general name for a group of fat-soluble pigments found in green, yellow, and leafy vegetables, and yellow fruits. They are aliphatic hydrocarbons containing 4 terpene subunits. Carotenes,Carotenoid,Tetraterpene Derivatives,Tetraterpenes,Carotene,Derivatives, Tetraterpene
D005638 Fruit The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds. Berries,Legume Pod,Plant Aril,Plant Capsule,Aril, Plant,Arils, Plant,Berry,Capsule, Plant,Capsules, Plant,Fruits,Legume Pods,Plant Arils,Plant Capsules,Pod, Legume,Pods, Legume
D000072669 Gene Editing Genetic engineering or molecular biology techniques that involve DNA REPAIR mechanisms for incorporating site-specific modifications into a cell's genome. Base Editing,Genome Editing,Editing, Base,Editing, Gene,Editing, Genome
D000073658 Loss of Function Mutation A mutation that causes a decrease in or elimination of a gene product's activity. Leaky Mutation,Null Mutation,Leaky Mutations,Mutation, Leaky,Mutation, Null,Mutations, Leaky,Mutations, Null,Null Mutations
D000076987 CRISPR-Associated Protein 9 An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS PYOGENES and other bacteria where it participates in an adaptive immune function to cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand, and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6 nucleotide DNA sequence immediately following the sequence targeted by Cas9. Cas9 Endonuclease,Cas9 Enzyme,Cas9 Protein,CRISPR Associated Protein 9,Endonuclease, Cas9,Enzyme, Cas9
D053858 Metabolic Networks and Pathways Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites. Metabolic Networks,Metabolic Pathways,Metabolic Network,Metabolic Pathway,Network, Metabolic,Networks, Metabolic,Pathway, Metabolic,Pathways, Metabolic
D056949 Phototropins Blue-light receptors that regulate a range of physiological responses in PLANTS. Examples include: PHOTOTROPISM, light-induced stomatal opening, and CHLOROPLAST movements in response to changes in light intensity. Phototropin
D018551 Solanum lycopersicum A plant species of the family SOLANACEAE, native of South America, widely cultivated for their edible, fleshy, usually red fruit. Lycopersicon esculentum,Tomatoes,Tomato
D064113 CRISPR-Cas Systems Adaptive antiviral defense mechanisms, in archaea and bacteria, based on DNA repeat arrays called CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS (CRISPR elements) that function in conjunction with CRISPR-ASSOCIATED PROTEINS (Cas proteins). Several types have been distinguished, including Type I, Type II, and Type III, based on signature motifs of CRISPR-ASSOCIATED PROTEINS. CRISPR Cas Systems,CRISPR-Cas System,System, CRISPR-Cas,Systems, CRISPR-Cas

Related Publications

Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
April 2014, Plant physiology,
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
May 2016, International journal of molecular sciences,
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
June 2014, Clinical medicine (London, England),
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
December 2023, The New phytologist,
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
November 1981, Hospital supervisor's bulletin,
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
December 2020, United European gastroenterology journal,
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
June 1996, Nursing spectrum (D.C./Baltimore metro ed.),
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
December 1999, Healthcare hazardous materials management : HHMM,
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
January 2000, Tropical gastroenterology : official journal of the Digestive Diseases Foundation,
Himabindu Vasuki Kilambi, and Alekhya Dindu, and Kapil Sharma, and Narasimha Rao Nizampatnam, and Neha Gupta, and Nikhil Padmanabhan Thazath, and Ajayakumar Jaya Dhanya, and Kamal Tyagi, and Sulabha Sharma, and Sumit Kumar, and Rameshwar Sharma, and Yellamaraju Sreelakshmi
July 2022, Immunotherapy,
Copied contents to your clipboard!