Biomaterial Database

Effects of cholecystokinin octapeptide on contractile motility of guinea pig common bile duct. 1988

T Takahashi, and M Kusunoki, and M Kantoh, and T Yamamura, and J Utsunomiya

Second Department of Surgery, Hyogo College of Medicine, Japan.

Cholecystokinin octapeptide (CCK-8)-induced contraction and [3H]acetylcholine ([3H]-ACh) release from the longitudinal muscle strip of the guinea pig common bile duct were studied by a standard organ bath method and superfusion system. CCK-8 (10(-7) M) caused a biphasic contraction, a rapid phasic contraction followed by a fading tonic contraction. Hexamethonium, propranolol, and phentolamine had no effects on CCK-8-evoked contraction. Atropine or tetrodotoxin significantly reduced CCK-8-evoked initial phasic contraction and produced a rightward shift of the dose-response curve for CCK-8 (10(-10)-3 x 10(-7) M). CCK-8 provoked a significant [3H]ACh release in a dose-dependent manner, which was almost completely abolished by tetrodotoxin or Ca2+-free medium. These results suggest that CCK-8 receptors are present not only on the smooth muscle cells but also on the intramural cholinergic nerve cell bodies of the guinea pig common bile duct.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009130	Muscle, Smooth	Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)	Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D002217	Carbachol	A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS.	Carbamylcholine,Carbacholine,Carbamann,Carbamoylcholine,Carbastat,Carbocholine,Carboptic,Doryl,Isopto Carbachol,Jestryl,Miostat,Carbachol, Isopto
D003135	Common Bile Duct	The largest bile duct. It is formed by the junction of the CYSTIC DUCT and the COMMON HEPATIC DUCT.	Choledochus,Bile Duct, Common,Common Bile Ducts,Duct, Common Bile
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004558	Electric Stimulation	Use of electric potential or currents to elicit biological responses.	Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D005260	Female		Females
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia