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X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation. 2021
Archana Bhartiya, and
Darren Batey, and
Silvia Cipiccia, and
Xiaowen Shi, and
Christoph Rau, and
Stanley Botchway, and
Mohammed Yusuf, and
Ian K Robinson
London Centre for Nanotechnology, University College, London, UK.
Studies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced 'X-ray karyotypes' of both heavy metal-stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation doses due to radiation damage. The experiments were performed at the I-13 beamline, Diamond Light Source, Didcot, UK, using the phase-sensitive X-ray ptychography method.
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