-transparent.png){kind=logo}
In seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. The nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. These are characteristics of biologically active estrogen receptors. Pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six cell strains, indicating an induction of functional progesterone receptors. RNA blot analysis demonstrated the presence of messenger RNA for the human estrogen receptor. The data suggest that estrogen acts directly on human bone cells through a classical estrogen receptor-mediated mechanism.
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
September 1993,
Biochemical and biophysical research communications,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
January 2000,
Cell biology international,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
June 1991,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
January 1990,
European journal of cancer (Oxford, England : 1990),
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
May 2004,
Calcified tissue international,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
February 1996,
Journal of cellular biochemistry,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
August 1991,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
March 1991,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
July 1992,
Biochemical and biophysical research communications,
E F Eriksen, and
D S Colvard, and
N J Berg, and
M L Graham, and
K G Mann, and
T C Spelsberg, and
B L Riggs
May 1992,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
