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A protocol is described for cytogenetic assays of chemical mutagens using mammalian cells in vitro. The system employs continuous drug treatment (3 concentrations) for up to 8 h and recovery-cell populations after pulse treatments with a high dose. Both direct fixation (for recording spindle anomalies in anaphase) and colcemid-hypotonic fixation (for reading metaphase chromosome aberrations) are used in order to estimate the effects of an agent as a mitotic poison and as a clastogen respectively. Some DNA intercalating dyes (acridine orange, quinacrine mustard, neutral red) were found to be highly clastogenic whereas others (quinacrine dihydrochloride, 33258 Hoechst) are not.
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
October 1987,
Mutation research,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
November 1954,
Annals of the New York Academy of Sciences,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
January 1985,
Human genetics,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
May 1988,
Mutagenesis,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
January 2012,
Methods in molecular biology (Clifton, N.J.),
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
January 2008,
TSitologiia i genetika,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
May 2001,
Current protocols in toxicology,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
January 1986,
Environmental mutagenesis,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
March 1993,
Mutation research,
T C Hsu, and
C J Collie, and
A F Lusby, and
D A Johnston
January 1978,
Journal of environmental pathology and toxicology,
