-transparent.png){kind=logo}
A reproducible method is described for the separation and quantification of ascorbic acid and dehydroascorbic acid by ion-pairing reverse-phase high performance liquid chromatography and detection by absorbance at 232 nm. Lowest detectable concentrations with a linear response of detection were 5 nmol for ascorbic acid and 50 nmol for dehydroascorbic acid. This method was applied to the analysis of C3H/10T1/2 cells and culture medium after influx or efflux experiments and single or multiple treatments with ascorbic acid. Subsequent measurement of the radioactivity in the eluted fractions increased the detectability of both ascorbic acid and dehydroascorbic acid to 10 to 20 pmol.
L L Ibric, and
W F Benedict, and
A R Peterson
December 1991,
The American journal of clinical nutrition,
L L Ibric, and
W F Benedict, and
A R Peterson
January 1992,
Journal of pharmaceutical and biomedical analysis,
L L Ibric, and
W F Benedict, and
A R Peterson
July 1976,
Cancer research,
L L Ibric, and
W F Benedict, and
A R Peterson
August 1995,
Clinical chemistry,
L L Ibric, and
W F Benedict, and
A R Peterson
April 1991,
Journal of chromatography,
L L Ibric, and
W F Benedict, and
A R Peterson
May 1995,
Journal of chromatography. B, Biomedical applications,
L L Ibric, and
W F Benedict, and
A R Peterson
January 1985,
Basic life sciences,
L L Ibric, and
W F Benedict, and
A R Peterson
January 1989,
The Analyst,
L L Ibric, and
W F Benedict, and
A R Peterson
February 1985,
Radiation research,
L L Ibric, and
W F Benedict, and
A R Peterson
October 1985,
International journal of cancer,
