Biomaterial Database

In Vitro Characterization of Poly(Lactic Acid)/ Poly(Hydroxybutyrate)/ Thermoplastic Starch Blends for Tissue Engineering Application. 2021

Martina Culenova, and Ivana Birova, and Pavol Alexy, and Paulina Galfyova, and Andreas Nicodemou, and Barbora Moncmanova, and Roderik Plavec, and Katarina Tomanova, and Premysl Mencik, and Stanislav Ziaran, and Lubos Danisovic

Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, 811 08 Bratislava, Slovak Republic.

Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.

UI	MeSH Term	Description	Entries
D011091	Polyesters	Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.	Polyester
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006885	Hydroxybutyrates	Salts and esters of hydroxybutyric acid.	Hydroxybutyric Acid Derivatives,Hydroxybutyric Acids,Acid Derivatives, Hydroxybutyric
D023822	Tissue Engineering	Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.	Engineering, Tissue
D066330	Printing, Three-Dimensional	Process for making, building or constructing a physical object from a three-dimensional digital model by laying down many successive thin layers of building material.	3-D Printing,3-Dimensional Printing,3D Printing,Three-Dimensional Printing,3 D Printing,3 Dimensional Printing,3-D Printings,3-Dimensional Printings,3D Printings,Printing, 3-D,Printing, 3-Dimensional,Printing, 3D,Printing, Three Dimensional,Printings, 3-D,Printings, 3-Dimensional,Printings, 3D,Printings, Three-Dimensional,Three Dimensional Printing,Three-Dimensional Printings