Duckweed (DW) is a highly small, free-floating aquatic plant. It grows and reproduces rapidly, comprises mainly protein and carbohydrate, and has substantial potential as a feedstock to produce bioplastics due to its renewability and having very little impact on the food chain. The aim of this work was to analyze the effect of DW biomass on the characteristics and properties of bio-based and biodegradable plastics based on a poly(lactic acid)/thermoplastic cassava starch (PLA/TPS) blend. Various amounts of DW biomass were compounded with PLA and TPS in a twin-screw extruder and then converted into dumbbell-shaped specimens using an injection molding machine. The obtained PLA/TPS blends filled with DW biomass exhibited a lower melt flow ability, higher moisture content, and increased surface hydrophilicity than the neat PLA/TPS blend. Incorporation of DW with low concentrations of 2.3 and 4.6 wt% increased the tensile strength, Young's modulus, and hardness of the PLA/TPS blend. Moisture and glycerol from DW and TPS played important roles in reducing the Tg, Tcc, Tm, and Td of PLA in the blends. The current work demonstrated that DW could be used as a biofiller for PLA/TPS blends, and the resulting PLA/TPS blends filled with DW biomass have potential in manufacturing injection-molded articles for sustainable, biodegradable, and short-term use.