As we approach the twentieth anniversary of completing the international Human Genome Project, the next (and arguably most significant) frontier in biology consists of functionally understanding the proteins, which are encoded by the genome and play a crucial role in all of biology and medicine. To accomplish this challenge, different proteomics strategies must be devised to examine the activities of gene products (proteins) at scale. Among them, protein microarrays have been used to accomplish a wide variety of investigations such as examining the binding of proteins and proteoforms to DNA, small molecules, and other proteins; characterizing humoral immune responses in health and disease; evaluating allergenic proteins; and profiling protein patterns as candidate disease-specific biomarkers. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers involved in the field of protein microarrays provide concise descriptions of the methodologies that they currently use to fabricate microarrays and how they apply them to analyze protein interactions and responses of proteins to dissect human disease.