-transparent.png){kind=logo}
The biosynthesis of human preproparathyroid hormone (hpreproPTH) and the processing to mature parathyroid hormone (hPTH) was investigated in yeast. Cells were transformed with a plasmid that carried a fusion gene made of the yeast pyruvate kinase promoter, complementary DNA (cDNA) encoding a slightly modified form of hpreproPTH and the transcription termination signal from yeast triosephosphate-isomerase. In transformed yeast cells we identified a protein that was recognized by a PTH antiserum and, on gel electrophoresis, comigrated with hpreproPTH marker. The amino-terminal sequence of the protein was consistent with that of hpreproPTH, indicating that the hormone precursor is not processed. It was localized inside the cell, when analyzed in pulse-chase experiments by trypsin accessibility in intact and lysed spheroplasts. In contrast, when mRNA from these yeast cells and from human parathyroid tissue was translated into preproPTH in a reticulocyte lysate supplemented with canine pancreatic microsomes, the preproPTHs from both mRNAs were transported and cleaved with identical efficiencies. We conclude that hpreproPTH is synthesized in yeast but not recognized and processed like a precursor of a secreted protein by the yeast secretory apparatus.
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
December 1988,
The Journal of biological chemistry,
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
September 1987,
Molecular endocrinology (Baltimore, Md.),
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
December 1981,
Proceedings of the National Academy of Sciences of the United States of America,
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
February 1989,
Molecular endocrinology (Baltimore, Md.),
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
December 1988,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
December 1994,
Biochimica et biophysica acta,
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
October 1979,
Proceedings of the National Academy of Sciences of the United States of America,
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
February 2002,
American journal of veterinary research,
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
April 1988,
The Journal of endocrinology,
W Born, and
M Freeman, and
W Bornstein, and
A Rapoport, and
R D Klein, and
G N Hendy, and
H G Khorana, and
A Rich, and
J T Potts, and
H M Kronenberg
March 1984,
The Journal of biological chemistry,
