Biomaterial Database

Characteristics of a neutral amino acid transport system (system A) in osteoblastic rat osteosarcoma cells. 1987

B J Baum, and A Shteyer

Transport of alpha-aminoisobutyric acid (AIB) in the clonal, osteoblastic-like cell line, ROS 17/2, was characterized. AIB transport was time-, temperature- and Na+-dependent. Both ouabain and monensin inhibited AIB transport in these cells. AIB uptake followed Michaelis-Menten kinetics with an apparent Km = 0.57 mM and a Vmax = 4.07 nmol/30 min/plate. These characteristics are consistent with the presence of system A neutral amino acid transport in ROS 17/2 cells. Exposure of ROS 17/2 cells to either parathyroid hormone or dibutyryl cyclic AMP (db-cAMP), but not to dibutyryl cyclic GMP (db-cGMP), markedly stimulated AIB transport. This suggests that extracellular stimuli which enhance osteogenic responses in this cell type, coordinately upregulate system A transport.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D002250	Carbon Radioisotopes	Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.	Radioisotopes, Carbon
D000621	Aminoisobutyric Acids	A group of compounds that are derivatives of the amino acid 2-amino-2-methylpropanoic acid.	Acids, Aminoisobutyric
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic
D012516	Osteosarcoma	A sarcoma originating in bone-forming cells, affecting the ends of long bones. It is the most common and most malignant of sarcomas of the bones, and occurs chiefly among 10- to 25-year-old youths. (From Stedman, 25th ed)	Sarcoma, Osteogenic,Osteogenic Sarcoma,Osteosarcoma Tumor,Osteogenic Sarcomas,Osteosarcoma Tumors,Osteosarcomas,Sarcomas, Osteogenic,Tumor, Osteosarcoma,Tumors, Osteosarcoma
D012964	Sodium	A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.	Sodium Ion Level,Sodium-23,Ion Level, Sodium,Level, Sodium Ion,Sodium 23
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus