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The accumulation and output of 3H -prostaglandins (PGs), E2 and F2 alpha, into and from uterine strips isolated from ovariectomized rats, either in presence or in absence of exogenous progesterone, were explored. Tissue-to-medium ratio of 3H-counts (T/M-ratio), was determined. The same was done in solutions containing 14C-sucrose. During a 60 min incubation period in a solution containing 3H -PGF2 alpha, a net accumulation of radioactivity was evident in control (no progesterone) uterine slices. The T/M-ratio for 3H-PGF2 alpha, increased with time, reaching maximal values at 45 min. Progesterone (100 ug.ml-1) attenuated the uptake process, as evidenced by stable values of T/M-ratio, as time progressed. On the other hand, control T/M-ratio for 3H-PGE2, although similar to that for 3H -PGF2 alpha, was not influenced by the presence of exogenous progesterone. Regarding labelled PG release from the tissue, it was observed that, during an experimental period of 60 min, most tritium from control slices was released within the first 30 min after incubation with 3H -PGF2 alpha, whereas, following the presence and subsequent removal of exogenous progesterone, the bulk of 3H -released took place at 6-70 min. On the other hand, the release of 3H after an incubation with 3H -PGE2, was also maximal as that for 3H -PGF2, alpha within the first 30 min and resulted not altered after a period of exposure and removal of progesterone. The foregoing results suggest an specific pharmacological effect of progesterone, attenuating the uptake and retarding the outflow of PGF2 alpha, but not that of PGE2, into and from uterine slices of ovariectomized rats. Findings reported herein are discussed in terms of progesterone priming and withdrawal, in relation to PGF2 alpha fluxes in the rat uterus during the sex cycle, as well as in relation to PG binding to tissue receptors.
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
September 1992,
Prostaglandins, leukotrienes, and essential fatty acids,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
December 1991,
Prostaglandins, leukotrienes, and essential fatty acids,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
October 1985,
Prostaglandins, leukotrienes, and medicine,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
October 1982,
Prostaglandins, leukotrienes, and medicine,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
March 1986,
Biochemical and biophysical research communications,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
July 1989,
The Biochemical journal,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
January 1987,
Archives of oral biology,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
February 1991,
The Biochemical journal,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
June 1984,
Thorax,
M F Gimeno, and
A M Franchi, and
E T González, and
A L Gimeno
October 1981,
Pharmacology, biochemistry, and behavior,
