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Transcription of the rat growth hormone (rGH) gene in pituitary cells is increased by addition of thyroid hormone (T3). This induction is dependent on the presence of specific sequences just upstream of the rGH promoter. We have partially purified T3 receptor from rat liver and examined its interaction with these rGH sequences. We show here that T3 receptor binds specifically to a site just upstream of the basal rGH promoter. This binding site includes two copies of a 7-base-pair direct repeat, the centers of which are separated by 10 base pairs. Deletions that specifically remove the T3 receptor binding site drastically reduce response to T3 in transient transfection experiments. These results demonstrate that T3 receptor can recognize specific DNA sequences and suggest that it can act directly as a positive transcriptional regulatory factor.
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R L Warne, and
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D D Moore
April 1992,
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R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
July 1988,
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R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
November 1986,
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R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
April 1992,
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R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
April 1986,
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R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
March 1994,
Molecular endocrinology (Baltimore, Md.),
R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
November 1992,
FEBS letters,
R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
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R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
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R J Koenig, and
G A Brent, and
R L Warne, and
P R Larsen, and
D D Moore
January 1987,
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