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We have isolated the individual gamma-crystallins expressed in young human lenses and identified with which of the six known human gamma-crystallin genes they each correspond. We find that at least 90% of the gamma-crystallins synthesized in the young human lens are the products of genes gamma G3 and gamma G4. We demonstrate that gamma G4-crystallin undergoes a temperature-dependent phase separation, and we have measured the low-concentration branch of its coexistence curve (phase separation temperature vs. concentration) up to about 40 mg/ml. By comparison, we found no evidence of gamma G3-crystallin phase separating, even at lower temperatures and higher concentrations. This is consistent with predictions based on sequence homology between human and rat gamma-crystallins. The implications of these findings for human inherited and senile cataracts are considered.
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
January 2016,
Biochimica et biophysica acta,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
April 1979,
International journal of peptide and protein research,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
October 1991,
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R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
July 1982,
Experimental eye research,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
September 1998,
Experimental eye research,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
May 1984,
Proceedings of the National Academy of Sciences of the United States of America,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
July 1984,
Current eye research,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
December 2002,
Biochemistry,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
January 1983,
Nippon Ganka Gakkai zasshi,
R J Siezen, and
J A Thomson, and
E D Kaplan, and
G B Benedek
December 1995,
Yan ke xue bao = Eye science,
