BIOMAT Database Logo BIOMAT Database Logo

Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin. 2021

Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Biodiscovery Institute, School of Pharmacy, University of Nottingham, University Park, Nottingham, United Kingdom.

As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07T). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster.

UI MeSH Term Description Entries
D000082403 Actinomadura A genus of gram-positive bacteria in the family Thermomonosporaceae, order ACTINOMYCETALES. It is a filamentous bacterium found in soil and is a causative agent for MADURA FOOT. Excellospora,Streptomycoides
D001482 Base Composition The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid. Base Ratio,G+C Composition,Guanine + Cytosine Composition,G+C Content,GC Composition,GC Content,Guanine + Cytosine Content,Base Compositions,Base Ratios,Composition, Base,Composition, G+C,Composition, GC,Compositions, Base,Compositions, G+C,Compositions, GC,Content, G+C,Content, GC,Contents, G+C,Contents, GC,G+C Compositions,G+C Contents,GC Compositions,GC Contents,Ratio, Base,Ratios, Base
D047028 Macrocyclic Compounds Cyclic compounds with a ring size of approximately 1-4 dozen atoms. Macrocycle Compounds,Spherands,Torands,Compounds, Macrocycle,Compounds, Macrocyclic
D023181 Antimicrobial Cationic Peptides Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane. Cationic Antimicrobial Peptide,Cationic Antimicrobial Peptides,Cationic Host Defense Peptides,Host Defense Peptide,Microbicidal Cationic Proteins,Amphipathic Cationic Antimicrobial Peptides,Host Defense Peptides,Antimicrobial Peptide, Cationic,Antimicrobial Peptides, Cationic,Cationic Peptides, Antimicrobial,Cationic Proteins, Microbicidal,Defense Peptide, Host,Defense Peptides, Host,Peptide, Cationic Antimicrobial,Peptide, Host Defense,Peptides, Antimicrobial Cationic,Peptides, Cationic Antimicrobial,Peptides, Host Defense,Proteins, Microbicidal Cationic

Related Publications

Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Actinomadura roseirufa sp. nov., producer of semduramicin, a polyether ionophore.
October 2019, International journal of systematic and evolutionary microbiology,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Actinomadura mexicana sp. nov. and Actinomadura meyerii sp. nov., two novel soil sporoactinomycetes.
November 2003, Systematic and applied microbiology,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.
July 2008, International journal of systematic and evolutionary microbiology,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Description of the bacterial RNA polymerase inhibitor GE23077-producer Actinomadura sp. NRRL B-65521T as Actinomadura lepetitiana sp. nov.
August 2020, International journal of systematic and evolutionary microbiology,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Actinomadura jiaoheensis sp. nov. and Actinomadura sporangiiformans sp. nov., two novel actinomycetes isolated from muddy soil and emended description of the genus Actinomadura.
December 2015, Antonie van Leeuwenhoek,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Actinomadura namibiensis sp. nov.
May 2003, International journal of systematic and evolutionary microbiology,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Cationomycin, a new polyether ionophore antibiotic produced by Actinomadura Nov. sp.
November 1981, The Journal of antibiotics,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Actinomadura logoneensis sp. nov., a novel actinomycete isolated from the soil.
September 2019, International journal of systematic and evolutionary microbiology,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
[New species of Actinomadura recticatena sp. nov. and its antibiotic properties].
January 1984, Antibiotiki,
Francesco Saverio Tarantini, and Mara Brunati, and Anna Taravella, and Lucia Carrano, and Francesco Parenti, and Kar Wai Hong, and Paul Williams, and Kok Gan Chan, and Stephan Heeb, and Weng C Chan
Actinomadura amylolytica sp. nov. and Actinomadura cellulosilytica sp. nov., isolated from geothermally heated soil.
July 2015, Antonie van Leeuwenhoek,
Copied contents to your clipboard!