-transparent.png){kind=logo}
Alpha 1-antitrypsin (alpha 1AT) present in large amounts in human serum and synthesized predominantly in hepatocytes, is the most abundant protease inhibitor and alpha 1AT mutant proteins are associated with hereditary disorders. To investigate the regulation of the normal human alpha 1AT gene, we have microinjected fertilized mouse eggs with a 17.5 kb DNA fragment containing the entire gene with 7 kb 5' and 0.3 kb 3' flanking sequences. We show that this DNA fragment contains all the information for efficient, accurate and tissue-specific expression. High serum concentration of the human protein was found in three independent transgenic mouse lines. The human alpha 1AT RNA is transcribed efficiently in liver, kidney and macrophages and we demonstrate that two different promoters are used for the expression in liver and macrophages of the transgenic mice.
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
February 1987,
Nucleic acids research,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
March 1989,
DNA (Mary Ann Liebert, Inc.),
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
April 1987,
Genes & development,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
August 1996,
Biochemical Society transactions,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
September 1989,
The Journal of biological chemistry,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
June 1989,
The Journal of biological chemistry,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
July 1995,
Biulleten' eksperimental'noi biologii i meditsiny,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
October 1987,
Nucleic acids research,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
September 1987,
The EMBO journal,
U Rüther, and
M Tripodi, and
R Cortese, and
E F Wagner
March 1996,
Biochimica et biophysica acta,
