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Spleen cells derived from BN rats receiving HgCl2 were fused with the nonsecreting rat myeloma cell line IR983F. We screened 59 supernatants from immunoglobulin-secreting hybrids for antibody activity against actin, tubulin, autologous and heterologous myosin, myoglobin, dsDNA, peroxidase, and the haptens TNP, NIP, NNP, and NBrP. Six monoclonal antibodies (mAb) were found to react with antigen(s) of the panel. At least three groups of antibody specificities were identified: clones reacting with TNP (1 IgM, 1 IgE); clones reacting with horseradish peroxidase (1 IgM); and clones possessing widespread reactivity for several antigens as found for mouse natural autoantibodies (2 IgM, 1 IgE). We also analyzed the idiotypic (Id) determinants of the 59 mAb by using anti-Id antibodies described elsewhere prepared in rabbits against the BALB/c D23 natural monoclonal autoantibody and recognizing a BALB/c recurrent Id (Id D23) of natural polyspecific autoantibodies. We found that all rat mAb that possessed widespread reactivities bore this Id. We performed similar studies in sera from normal and mercury-stimulated rats. The results indicate a role for HgCl2 in the stimulation of natural antibodies producing cells and the existence of interspecies cross-reactive Id among mouse and rat natural antibodies.
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
May 1986,
Journal of immunology (Baltimore, Md. : 1950),
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
April 1990,
Journal of immunology (Baltimore, Md. : 1950),
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
November 1995,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
November 1994,
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
January 1978,
Annales d'immunologie,
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
June 1982,
Clinical and experimental immunology,
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
December 1993,
Clinical immunology and immunopathology,
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
March 2006,
Rheumatology international,
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
September 1988,
Clinical and experimental immunology,
P Lymberi, and
F Hirsch, and
J Kuhn, and
T Ternynck, and
P Druet, and
S Avrameas
November 1982,
Journal of immunology (Baltimore, Md. : 1950),
