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Glucose-6-phosphate dehydrogenase (G6PD) is an ubiquitous enzyme which by determining the NADPH level has a crucial role in NADPH-mediated reductive processes in all cells (1). The structural gene for G6PD, Gd, is X-linked in mammals and on the basis of its expression in many tissues, it can be regarded as a typical "housekeeping" gene (2). Over 300 variants of the protein are known, many of which have deficient enzyme activity. Nearly 100 of these variants are polymorphic in various populations (3). The mammalian enzyme is a homodimer or a homotetramer with a subunit molecular weight of approximately 56000 daltons (4). Here we report the isolation of cDNA clones from HeLa cells, SV40-transformed human fibroblasts, human placenta and human teratocarcinoma cell lines. These clones have enabled us to sequence the entire coding region of Gd. Thus, the entire amino acid sequence of human G6PD is provided for the first time. This work is the first step for structural analysis of G6PD variants and for an understanding of the biological features of this enzyme at the molecular level.
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
June 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
August 1987,
Proceedings of the National Academy of Sciences of the United States of America,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
March 1995,
Nihon rinsho. Japanese journal of clinical medicine,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
December 2004,
Nihon rinsho. Japanese journal of clinical medicine,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
September 1994,
Biochimica et biophysica acta,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
August 1977,
Journal of medical genetics,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
April 1987,
Biochemical genetics,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
April 1993,
DNA and cell biology,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
January 2006,
Clinical laboratory science : journal of the American Society for Medical Technology,
M G Persico, and
G Viglietto, and
G Martini, and
D Toniolo, and
G Paonessa, and
C Moscatelli, and
R Dono, and
T Vulliamy, and
L Luzzatto, and
M D'Urso
January 1979,
Dakar medical,
