Comparison of Cytokines Expression from Human Monocyte-Derived Macrophages Infected with Different Species of Mycobacteria. 2022

Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, and Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Cytokines have an important role in mounting effective host immune response against mycobacteria. Latent tuberculosis infection (LTBI) is an indication of containment of mycobacteria by the host immune response, whereas active TB is an indication of a failure of the immune response to contain Mycobacterium tuberculosis. The dynamics of this host-immune response during in vitro infection experiment is believed to be indicative of behavior in the LTBI and active-TB cases. This relationship is, however, not fully elucidated. We investigated the cytokines expression at mRNA and protein level across 2 different protocols, that is, an in vitro protocol comparing human monocyte-derived macrophages (hMDMs; n = 12) infected with different species of mycobacteria, and a clinical protocol comparing TB-Antigen-Nil specimens from LTBI (n = 12) and active-TB (n = 12) cases. We found that in vitro infection of hMDMs with Mycobacterium bovis Bacillus Calmette-Guérin (BCG) and M. tuberculosis R179 showed increased colony-forming units at all time points postinfection. M. bovis BCG-infected hMDMs demonstrated higher levels of 5 cytokines [interferon (IFN)-γ, interleukin (IL)-6, IL-1β, IL-12p40, and IL-12p70] at different intervals compared to M. tuberculosis R179. Compared to LTBI, active-TB cases had higher mRNA expression of IFN-α, IL-6, and IL-8, and lower expression of IFN-γ, IL-1α, IL-1β, IL-4, and tumor necrosis factor-α. Overall, we observed differential host responses at mRNA and protein levels during experimentally controlled in vitro infection, but no prominent differences were observed in the clinical protocol. Therefore, the result of the in vitro experiment model of cytokine response against mycobacteria should be interpreted cautiously when relating to LTBI and active-TB.

UI MeSH Term Description Entries
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D009169 Mycobacterium tuberculosis A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation. Mycobacterium tuberculosis H37Rv
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001500 BCG Vaccine An active immunizing agent and a viable avirulent attenuated strain of MYCOBACTERIUM BOVIS, which confers immunity to mycobacterial infections. It is used also in immunotherapy of neoplasms due to its stimulation of antibodies and non-specific immunity. Bacillus Calmette Guerin Vaccine,Calmette Guerin Bacillus Vaccine,Calmette's Vaccine,Calmette Vaccine,Calmettes Vaccine,Vaccine, BCG,Vaccine, Calmette's
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014376 Tuberculosis Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM TUBERCULOSIS. Koch's Disease,Kochs Disease,Mycobacterium tuberculosis Infection,Infection, Mycobacterium tuberculosis,Infections, Mycobacterium tuberculosis,Koch Disease,Mycobacterium tuberculosis Infections,Tuberculoses
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine
D055985 Latent Tuberculosis The dormant form of TUBERCULOSIS where the person shows no obvious symptoms and no sign of the causative agent (Mycobacterium tuberculosis) in the SPUTUM despite being positive for tuberculosis infection skin test. Latent Tuberculosis Infection,Infection, Latent Tuberculosis,Infections, Latent Tuberculosis,Latent Tuberculoses,Latent Tuberculosis Infections,Tuberculoses, Latent,Tuberculosis Infection, Latent,Tuberculosis Infections, Latent,Tuberculosis, Latent

Related Publications

Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
January 2017, Frontiers in cellular and infection microbiology,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
June 1999, The Journal of pathology,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
January 2014, PloS one,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
September 1996, The Journal of general virology,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
February 1996, Biochemical Society transactions,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
May 2023, Immunobiology,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
June 2018, Parasitology,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
January 2018, Virulence,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
April 2001, Drug and alcohol dependence,
Abhilasha Madhvi, and Hridesh Mishra, and Novel N Chegou, and Mbali N Mkhonza, and Sedzani Ndou, and Gerard Tromp, and Bienyameen Baker
December 2001, Cytokine,
Copied contents to your clipboard!