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We have investigated the possibility of obtaining integration and expression of a native human gene in transgenic mice. An 11-kilobase (kb) human chromosomal DNA fragment including the insulin gene (1430 base pairs) was microinjected into fertilized mouse eggs. This fragment was present in the genomic DNA of several developing animals. One transgenic mouse and its progeny were analyzed for expression of the foreign gene. Synthesis and release of human insulin was revealed by detection of the human C-peptide in the plasma and urine. Human insulin mRNA was found in pancreas but not in other tissues. These findings indicate that the 11-kb human DNA fragment carries the sequences necessary for tissue-specific expression of the insulin gene and the human regulatory sequences react to homologous signals in the mouse.
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
January 1986,
Nature,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
January 1987,
Comptes rendus des seances de la Societe de biologie et de ses filiales,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
January 1989,
Hormone research,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
January 1987,
Molecular and cellular biology,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
September 1996,
Differentiation; research in biological diversity,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
January 1987,
Hepatology (Baltimore, Md.),
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
August 2001,
Transgenic research,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
January 1993,
Diabetes,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
December 1996,
Annals of the New York Academy of Sciences,
D Bucchini, and
M A Ripoche, and
M G Stinnakre, and
P Desbois, and
P Lorès, and
E Monthioux, and
J Absil, and
J A Lepesant, and
R Pictet, and
J Jami
April 1994,
Gene,
