BACKGROUND Self-medication is common among medical students. Medical and dental students can also educate the population about the use of medicines. The aim of the study was to find the mean knowledge score of self-medication among first and second year medical and dental students at a medical college in Nepal. METHODS A descriptive cross-sectional study was carried out in a medical college from 1st to 28th February 2021 using a structured online questionnaire after obtaining approval from the Institutional Review Committee (approval number 077/78/32). Convenience sampling method was undertaken. The responses were analyzed using Statistical Package for Social Sciences version 26. RESULTS Out of 173 students the mean±SD knowledge score was 79.47±6.76. The mean knowledge score of the participants of age group 17-18, 19-20 and 21-22 years were 83.8, 79 and 79.1 respectively. The mean knowledge scores of the male and female participants were 80.2 and 78.6 respectively. Similarly, the knowledge scores of respondents from undergraduate medical and dental backgrounds were 80.1 and 77.7 respectively. CONCLUSIONS Knowledge of self-medication was high among the students. However, educational sessions to further improve and strengthen knowledge can be carried out to improve the knowledge.