Blood pressure measurement has a great implication in medicine. Every medical personnel should have a sound knowledge regarding blood pressure measurement. This study was conducted with the aim of determining the knowledge regarding blood pressure measurement among first and second year medical students of a medical college. A descriptive cross-sectional study was conducted by Department of Physiology of Nepalese Army Institute of Health Sciences from June to December 2021 after receiving the ethical approval from Institutional Review Committee with registration number 394. One hundred ninety-seven students from first and second year were included in the study using whole sampling technique. Data was entered and analysed using Microsoft Excel 2019 and Statistical Package for Social sciences version 16.0. Descriptive statistics like frequency, percentage for binary data and mean, standard deviation for continuous data were calculated. Among 197 students from first and second year, 175 (88.83%) had satisfactory knowledge regarding blood pressure measurement with score ≥8 in Objective Structured Practical Examination. Eighty five (85.86%) students from first year and 90 (91.84%) students from second year had scores ≥8, hence had satisfactory knowledge on blood pressure measurement. A total of 18 (18.18%) first year students achieved a score of 10, while 24 (24.49%) students of the second year scored 12, which were the modal score. The proportion of first and second year students having satisfactory knowledge regarding blood pressure measurement is higher in our study. blood pressure; knowledge; medical students.