-transparent.png){kind=logo}
Using OST6 and OST7 monoclonal antibodies against human osteosarcoma cells, a solid-phase radioimmunosandwich assay was developed to quantitate a human osterosarcoma-associated antigen in a total of 242 sera from healthy adults and patients with various diseases. The levels of the antigen in sera were high in patients with osteosarcoma and in children without tumorous diseases compared with healthy adults; however, the highest level of the antigen was found in patients with obstructive jaundice. The quantity of the antigen correlated with serum alkaline phosphatase (EC 3.1.3.1.) activity, and showed a strong correlation (correlation coefficient, 0.94) in 50 sera. Immunolocalization of enzyme activity assay using monoclonal antibodies was performed to ascertain whether the antigen had alkaline phosphatase activity. This assay proved that OST6, OST7, and OST15 monoclonal antibodies recognized serum alkaline phosphatase; furthermore, these monoclonal antibodies seemed to react with not only the bone isoenzyme but also the liver isoenzyme.
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
July 1991,
Japanese journal of cancer research : Gann,
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
December 1987,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
January 1991,
International orthopaedics,
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
October 1976,
Journal of the National Cancer Institute,
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
January 1983,
American journal of human genetics,
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
October 1991,
Biokhimiia (Moscow, Russia),
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
January 1994,
European journal of cancer (Oxford, England : 1990),
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
January 1983,
Clinical chemistry,
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
January 1989,
Andrologia,
C Tanaka, and
T Yamamuro, and
T Masuda, and
H Tanaka, and
M Matsumoto, and
Y Kotoura, and
T Tsuji, and
J Toguchida, and
M Miyama-Inaba, and
M Ueda
May 1989,
Journal of clinical microbiology,
