-transparent.png){kind=logo}
A beta-lactamase produced by a penicillin-resistant strain of Serratia marcescens was isolated and purified. The kcat. value for benzylpenicillin was about 5% of that observed for the best cephalosporin substrates. However, the low Km of the penam resulted in a high catalytic efficiency (kcat./Km) and the classification of the enzyme as a cephalosporinase might not be completely justified. It also exhibited a low but measurable activity against cefotaxime, cefuroxime, cefoxitin and moxalactam. Substrate-induced inactivation was observed both with a very good (cephalothin) or a very bad (moxalactam) substrate. The active site was labelled by beta-iodopenicillanate. Trypsin digestion produced a 19-residue active-site peptide whose sequence clearly allowed the classification of the enzyme as a class C beta-lactamase.
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
February 2002,
Acta crystallographica. Section D, Biological crystallography,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
January 1995,
Microbiology and immunology,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
December 2014,
Diagnostic microbiology and infectious disease,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
September 1995,
Antimicrobial agents and chemotherapy,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
March 1999,
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
December 1995,
The Journal of antibiotics,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
November 2000,
Antimicrobial agents and chemotherapy,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
November 1997,
Biological & pharmaceutical bulletin,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
April 2003,
Diagnostic microbiology and infectious disease,
B Joris, and
F De Meester, and
M Galleni, and
S Masson, and
J Dusart, and
J M Frère, and
J Van Beeumen, and
K Bush, and
R Sykes
June 1994,
Antimicrobial agents and chemotherapy,
