Zeolite 4A as a jammer of bacterial communication in Chromobacterium violaceum and Pseudomonas aeruginosa. 2022

Seyhan Ulusoy, and Ramadan B Akalin, and Halime Çevikbaş, and Avni Berisha, and Ayhan Oral, and Gülgün Boşgelmez-Tinaz
Department of Biology, Suleyman Demirel University, Isparta, 32260, Turkey.

Aim: To investigate the hypothesis that zeolites interfere with quorum-sensing (QS) systems of Chromobacterium violaceum and Pseudomonas aeruginosa by adsorbing N-acyl homoserine lactone (AHL) signal molecules. Methods: QS inhibition by zeolite 4A was investigated using an AHL-based bioreporter assay. The adsorption of the AHLs was evaluated by performing inductively coupled plasma-optical emission spectroscopy and confirmed by Monte Carlo and molecular dynamic simulations. Results: Zeolite 4A reduced the violacein production in C. violaceum by over 90% and the biofilm formation, elastase and pyocyanin production in P. aeruginosa by 87, 68 and 98%, respectively. Conclusion: Zeolite 4A disrupts the QS systems of C. violaceum and P. aeruginosa by means of adsorbing 3-oxo-C6-AHL and 3-oxo-C12-AHL signaling molecules and can be developed as a novel QS jammer to combat P. aeruginosa-related infections.

UI MeSH Term Description Entries
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D002861 Chromobacterium A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria occurring in soil and water. Its organisms are generally nonpathogenic, but some species do cause infections of mammals, including humans.
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D017641 Zeolites Zeolites. A group of crystalline, hydrated alkali-aluminum silicates. They occur naturally in sedimentary and volcanic rocks, altered basalts, ores, and clay deposits. Some 40 known zeolite minerals and a great number of synthetic zeolites are available commercially. (From Merck Index, 11th ed) Zeolite
D053038 Quorum Sensing A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria. Quorum Quenching,Quenching, Quorum,Quenchings, Quorum,Quorum Quenchings,Sensing, Quorum
D054742 Acyl-Butyrolactones Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring. Acyl-Butyrolactone,Acyl-Butyryl-Homoserine Lactone,Acyl-Homoserine Lactone,Acylated Homoserine Lactone,Butyrylhomoserine Lactone,Acyl-Butyryl-Homoserine Lactones,Acyl-Homoserine Lactones,Butyrylhomoserine Lactones,Acyl Butyrolactone,Acyl Butyrolactones,Acyl Butyryl Homoserine Lactone,Acyl Butyryl Homoserine Lactones,Acyl Homoserine Lactone,Acyl Homoserine Lactones,Homoserine Lactone, Acylated,Lactone, Acyl-Butyryl-Homoserine,Lactone, Acyl-Homoserine,Lactone, Acylated Homoserine,Lactone, Butyrylhomoserine,Lactones, Acyl-Butyryl-Homoserine,Lactones, Acyl-Homoserine,Lactones, Butyrylhomoserine
D018441 Biofilms Encrustations formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedded in an EXTRACELLULAR POLYMERIC SUBSTANCE MATRIX that is secreted by the microbes. They occur on body surfaces such as teeth (DENTAL DEPOSITS); inanimate objects, and bodies of water. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and anti-fouling agents. Biofilm

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