-transparent.png){kind=logo}
We have isolated a gene that is very rapidly induced at the transcriptional level by DIF--a low molecular weight, diffusible factor necessary for stalk cell differentiation in Dictyostelium cells developing in vitro. The gene encodes a protein containing an N-terminal signal peptide preceding approximately 70 tandem repeats of a highly conserved 24 amino acid sequence with a high cysteine content. These features suggest it is an extracellular structural protein. During normal development, the gene is maximally expressed in the slug, in which the mRNA is very highly enriched in prestalk over prespore cells. The gene is not detectably expressed until the tipped aggregate stage, several hours later than prespore genes, suggesting that prespore cell differentiation precedes prestalk cell differentiation. The demonstration that DIF induces a gene normally only expressed in the prestalk zone of the slug provides strong evidence that DIF is a Dictyostelium morphogen.
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
November 1998,
Experimental cell research,
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
September 1990,
Development (Cambridge, England),
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
June 1983,
Cell,
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
December 2001,
Development (Cambridge, England),
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
July 1990,
Developmental biology,
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
November 1986,
Proceedings of the National Academy of Sciences of the United States of America,
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
May 1994,
Differentiation; research in biological diversity,
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
January 2013,
The International journal of developmental biology,
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
December 1999,
Seminars in cell & developmental biology,
J G Williams, and
A Ceccarelli, and
S McRobbie, and
H Mahbubani, and
R R Kay, and
A Early, and
M Berks, and
K A Jermyn
May 1987,
Development (Cambridge, England),
