The use of 10-cm-long plastic tuberculin syringes for freezing and storage of human semen is introduced. This new method reduces the number of prefreezing and postthawing manipulations to a minimum. Postthawing motility, penetration of spermatozoa into cervical mucus, and results of the hamster ovum test were similar for spermatozoa stored-frozen in tuberculin syringes and for spermatozoa stored-frozen in french straws. The results of the postinsemination test performed in six women were slightly better with fresh semen than with semen stored-frozen in tuberculin syringes. Donor insemination with fresh semen resulted in eight pregnancies per 24 insemination cycles; with semen stored-frozen in tuberculin syringes five pregnancies were achieved in 22 insemination cycles.