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A simplified liquid nitrogen vapour method for the cryopreservation of human semen. 1982

R M Ing

A simple manually operated liquid nitrogen vapour freezing apparatus has been developed for the cryopreservation of human semen. A test method using this apparatus was evaluated by comparison with a conventional freezing method using an ultra cold (-70 degrees C) freezer. Twenty different unselected fresh semen samples were analysed, diluted with cryoprotective medium and then divided into equal numbers of straws prior to freezing by the two methods. A comparison of the average sperm motility before freezing and after thawing using these two methods demonstrated a statistically significant superiority of the simplified test method.

UI MeSH Term Description Entries
D008297 Male Males
D009584 Nitrogen An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D005615 Freezing Liquids transforming into solids by the removal of heat. Melting
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012662 Semen Preservation The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Frozen Semen,Sperm Preservation,Preservation, Semen,Preservation, Sperm,Semen, Frozen
D013081 Sperm Motility Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression. Motilities, Sperm,Motility, Sperm,Sperm Motilities

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