Biomaterial Database

Effect of storage on the creamatocrit and total energy content of human milk. 1987

A Silprasert, and W Dejsarai, and R Keawvichit, and K Amatayakul

Human milk samples were collected from mothers on the third post-partum day with a manual breast-pump and pooled. They were divided into aliquots and stored at room temperature (24-25 degrees C), and at 4 degrees, 37 degrees and -20 degrees C. The creamatocrit (CR) values decreased significantly after storage at room temperature (P less than 0.03), or at 37 degrees C (P less than 0.001) for 45 min and 30 min, respectively. Creamatocrit remained stable for 14 d at 4 degrees C, and for up to 28 d at -20 degrees C. However, freezing and thawing samples twice significantly decreased creamatocrit values (P less than 0.001). The total energy concentration of the samples stored at -20 degrees C was stable for 28 d. These results indicate that strict precautions must be taken when handling and storing human milk samples for analysis of lipid content.

UI	MeSH Term	Description	Entries
D008055	Lipids	A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)	Lipid
D008895	Milk, Human	Milk that is produced by HUMAN MAMMARY GLANDS.	Breast Milk,Human Milk,Milk, Breast
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011309	Preservation, Biological	The process of protecting various samples of biological material.	Biological Preservation,Preservation, Biologic,Biologic Preservation
D005260	Female		Females
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293	Adolescent	A person 13 to 18 years of age.	Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor