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The capacity of lymphocytes from 23 human subjects to metabolize the model carcinogen 2-acetylaminofluorene (AAF) was assessed. These cells metabolized AAF to its 1-, 7- and N-hydroxylated metabolites. Alpha-naphthoflavone and metyrapone exhibited IC50's for all pathways of 0.02 microM and 1 mM, respectively. These data indicate that the same form of cytochrome P450 or forms with similar IC50's are mediating these reactions. No significant difference was observed between lymphocytes from smokers versus non-smokers in the N-hydroxylation of AAF, the initial step in the metabolic activation of this carcinogen. However, lymphocytes from smokers were faster detoxifiers of AAF as seen in their increased capacity to 1- and 7-hydroxylate AAF compared to lymphocytes from non-smokers.
M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
December 1985,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
March 1993,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
August 1974,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
June 1985,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
January 1981,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
July 1973,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
January 1972,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
January 1963,
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M E McManus, and
K J Trainor, and
A A Morley, and
W Burgess, and
I Stupans, and
D J Birkett
January 1981,
Journal of toxicology and environmental health,
