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The protein synthesis patterns at various stages of the cell cycle of Chinese hamster ovary cells were examined by labelling cells with [35S]methionine and then separating the proteins by isoelectric focussing and two-dimensional, nonequilibrium pH gradient gel electrophoresis. We have observed a number of proteins which display quantitative differences in synthesis at specific cell cycle stages and of these the alpha- and beta-tubulins have been identified. A few proteins appear to be uniquely synthesized at specific times during the cell cycle. These include the histones and a modified version of them, which are synthesized only in S phase, and a pair of 21 kilodalton (kDa), pI 5.5 proteins, which appear only in late G2 and mitosis. We have also identified a 58-kDa, pI 7.5 protein which is present at all cell cycle stages except during late G2. This protein appears to have the same temporal properties as a 57-kDa protein called "cyclin" originally described in sea urchin embryos.
J T Westwood, and
R B Church, and
E B Wagenaar
January 2021,
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J T Westwood, and
R B Church, and
E B Wagenaar
January 1973,
Biophysik,
J T Westwood, and
R B Church, and
E B Wagenaar
June 1973,
The Journal of cell biology,
J T Westwood, and
R B Church, and
E B Wagenaar
July 1981,
Molecular and cellular biology,
J T Westwood, and
R B Church, and
E B Wagenaar
February 1982,
Proceedings of the National Academy of Sciences of the United States of America,
J T Westwood, and
R B Church, and
E B Wagenaar
January 2018,
Methods in molecular biology (Clifton, N.J.),
J T Westwood, and
R B Church, and
E B Wagenaar
January 1991,
Infection and immunity,
J T Westwood, and
R B Church, and
E B Wagenaar
January 1995,
Experimental cell research,
J T Westwood, and
R B Church, and
E B Wagenaar
February 1983,
Experimental cell research,
J T Westwood, and
R B Church, and
E B Wagenaar
June 1984,
Zhongguo yao li xue bao = Acta pharmacologica Sinica,
