Biomaterial Database

Bromcresol purple dye-binding methods underestimate albumin that is carrying covalently bound bilirubin. 1987

V Bush, and R G Reed

We used automated and manual versions of two commonly used dye-binding methods for albumin to quantify albumin that had been modified by covalent addition of bilirubin. Dye-binding methods based on reaction with bromcresol purple underestimated the modified albumin by as much as 29%. Dye-binding methods based on reaction with bromcresol green were unaffected by the presence of covalently attached bilirubin. Neither method was affected by the presence of noncovalently bound bilirubin.

UI	MeSH Term	Description	Entries
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D001961	Bromcresol Green	An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.	Tetrabromocresolsulfonphthalein,Bromocresol Green,Green, Bromcresol,Green, Bromocresol
D001962	Bromcresol Purple	An indicator and reagent. It has been used for several purposes including the determination of serum albumin concentrations	Bromocresol Purple,Purple, Bromcresol,Purple, Bromocresol
D003408	Cresols	Any of three isomeric crystalline aromatic methylphenols, also known as hydroxytoluenes.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001663	Bilirubin	A bile pigment that is a degradation product of HEME.	Bilirubin IX alpha,Bilirubin, (15E)-Isomer,Bilirubin, (4E)-Isomer,Bilirubin, (4E,15E)-Isomer,Bilirubin, Calcium Salt,Bilirubin, Disodium Salt,Bilirubin, Monosodium Salt,Calcium Bilirubinate,Hematoidin,delta-Bilirubin,Bilirubinate, Calcium,Calcium Salt Bilirubin,Disodium Salt Bilirubin,Monosodium Salt Bilirubin,Salt Bilirubin, Calcium,delta Bilirubin
D012709	Serum Albumin	A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.	Plasma Albumin,Albumin, Serum

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