Serum albumin assay with bromcresol purple dye. 1985

M T Parviainen, and A Harmoinen, and H Jokela

Serum albumin was assayed with bromcresol purple using routine clinical chemical photometric analysers. Bromcresol purple and bromcresol green dyes were compared, and albumin assays using these dyes were compared to those made by radial immunodiffusion and rocket immunoelectrophoresis. Bromcresol purple seems to be insensitive to all serum proteins except albumin, and this dye is correlated linearly with radial immunodiffusion and with rocket electrophoresis.

UI MeSH Term Description Entries
D007122 Immunoelectrophoresis A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D010783 Photometry Measurement of the various properties of light. Photometries
D001961 Bromcresol Green An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator. Tetrabromocresolsulfonphthalein,Bromocresol Green,Green, Bromcresol,Green, Bromocresol
D001962 Bromcresol Purple An indicator and reagent. It has been used for several purposes including the determination of serum albumin concentrations Bromocresol Purple,Purple, Bromcresol,Purple, Bromocresol
D003408 Cresols Any of three isomeric crystalline aromatic methylphenols, also known as hydroxytoluenes.
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012709 Serum Albumin A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules. Plasma Albumin,Albumin, Serum

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