Absorbance errors from using pieced-together rotors in the Roche Cobas-Bio centrifugal analyzer. 1987

W G Miller, and E K Armitage

Two Cobas-Bio analyzers were found to have significantly increased imprecision, owing to absorbance errors, when we used rotors pieced together from segments of four to 10 cuvets. The difference in CV between whole/unbroken and four-cuvet or 10-cuvet segment rotors was significant by Student's unpaired t-test (P = 0.038 and less than 0.0001, respectively) and by the Mann-Whitney U test (P less than 0.0001 for either). Pieced-together rotors were found to have plastic fragments in some cuvets, both adjacent to the break points and in other positions within the segment. There was no significant difference in delta A between adjacent cuvets at break positions or in between break positions by unpaired t-test (P = 0.214) or by the Mann-Whitney U test (P = 0.235). There was a significant difference by either test (P less than 0.0001) for the delta A between adjacent cuvets in broken rotors and those in whole/unbroken rotors. We conclude that random errors in absorbance are observed both in cuvets adjacent to break positions and in cuvets removed from the break point when pieced-together rotors are used.

UI MeSH Term Description Entries
D011786 Quality Control A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed) Control, Quality,Controls, Quality,Quality Controls
D001322 Autoanalysis Method of analyzing chemicals using automation. Autoanalyses
D013053 Spectrophotometry The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D013223 Statistics as Topic Works about the science and art of collecting, summarizing, and analyzing data that are subject to random variation. Area Analysis,Estimation Technics,Estimation Techniques,Indirect Estimation Technics,Indirect Estimation Techniques,Multiple Classification Analysis,Service Statistics,Statistical Study,Statistics, Service,Tables and Charts as Topic,Analyses, Area,Analyses, Multiple Classification,Area Analyses,Classification Analyses, Multiple,Classification Analysis, Multiple,Estimation Technic, Indirect,Estimation Technics, Indirect,Estimation Technique,Estimation Technique, Indirect,Estimation Techniques, Indirect,Indirect Estimation Technic,Indirect Estimation Technique,Multiple Classification Analyses,Statistical Studies,Studies, Statistical,Study, Statistical,Technic, Indirect Estimation,Technics, Estimation,Technics, Indirect Estimation,Technique, Estimation,Technique, Indirect Estimation,Techniques, Estimation,Techniques, Indirect Estimation

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