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We describe a method for measuring plasma cholinesterase (EC 3.1.1.8) and dibucaine and fluoride numbers by using the Cobas-Bio. Benzoyl choline chloride is used as substrate. Reaction conditions are the same as in the corresponding manual method. The reaction is stopped with physostigmine. Choline oxidase (EC 1.1.3.17) is coupled with 4-aminoantipyrene. In the presence of peroxidase (EC 1.11.1.7), the 2-hydroxy-3,5-dichlorobenzenesulfonate indicator reaction gives a red product, measured at 505 nm. The analyzer is used in the "Multi Run" mode, with plasma cholinesterase and dibucaine and fluoride inhibition concurrently measured for eight samples. Coefficients of correlation between the manual and present method for plasma cholinesterase and dibucaine and fluoride numbers in 40 patients of various phenotypes were 0.843, 0.923, and 0.717, respectively. The inter-batch CV for each of the three assays was 2% to 3%.
S Ratnaike, and
F Gray, and
D Deam
July 1988,
Analytical biochemistry,
S Ratnaike, and
F Gray, and
D Deam
December 1983,
Clinical biochemistry,
S Ratnaike, and
F Gray, and
D Deam
June 1983,
Clinical chemistry,
S Ratnaike, and
F Gray, and
D Deam
September 1987,
Clinical chemistry,
S Ratnaike, and
F Gray, and
D Deam
April 1982,
Clinical chemistry,
S Ratnaike, and
F Gray, and
D Deam
March 1988,
American journal of clinical pathology,
S Ratnaike, and
F Gray, and
D Deam
March 1983,
Clinical chemistry,
S Ratnaike, and
F Gray, and
D Deam
December 1983,
American journal of clinical pathology,
S Ratnaike, and
F Gray, and
D Deam
February 1984,
Clinical chemistry,
S Ratnaike, and
F Gray, and
D Deam
October 1982,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
