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An enzymic rate method for measuring beta-hydroxybutyrate in plasma by use of beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) was adapted to the Roche COBAS-BIO centrifugal analyzer. Optimum reagent composition and reaction conditions were determined, to provide increased sensitivity and accuracy. Use of magnesium ions increased sensitivity. Comparison of results with those of a manual enzymic endpoint method involving perchloric acid protein-free filtrates of whole blood showed excellent correlation (r = 0.996). The method has good within-run and day-to-day precision. Linearity of the standard curve extends to 5000 mumol of beta-hydroxybutyrate per liter. The assay can be completed in less than 5 min and requires 15 microL of plasma and minimal reagent volumes.
J J Moore, and
M Marcus, and
S M Sax
September 1987,
Clinical chemistry,
J J Moore, and
M Marcus, and
S M Sax
June 1983,
Clinical chemistry,
J J Moore, and
M Marcus, and
S M Sax
August 1987,
Clinical chemistry,
J J Moore, and
M Marcus, and
S M Sax
March 1983,
Clinical chemistry,
J J Moore, and
M Marcus, and
S M Sax
April 1985,
Clinical chemistry,
J J Moore, and
M Marcus, and
S M Sax
July 1988,
Analytical biochemistry,
J J Moore, and
M Marcus, and
S M Sax
December 1983,
Clinical biochemistry,
J J Moore, and
M Marcus, and
S M Sax
September 1988,
Clinical chemistry,
J J Moore, and
M Marcus, and
S M Sax
February 1985,
Clinical biochemistry,
J J Moore, and
M Marcus, and
S M Sax
January 1981,
Journal of analytical toxicology,
