Overexpression of ZmSRG7 Improves Drought and Salt Tolerance in Maize (Zea mays L.). 2022

Xiaotong Wei, and Xuhong Fan, and Honglin Zhang, and Peng Jiao, and Zhenzhong Jiang, and Xuan Lu, and Siyan Liu, and Shuyan Guan, and Yiyong Ma
College of Agronomy, Jilin Agricultural University, Changchun 130118, China.

Osmotic stress caused by drought and high salinity is the key factor limiting plant growth. However, its underlying molecular regulatory mechanism remains unclear. In this study, we found the stress-related gene Zm00001d019704 (ZmSRG7) based on transcriptome sequencing results previously obtained in the laboratory and determined its biological function in maize. We found that ZmSRG7 was significantly expressed in both roots and leaves under 10% PEG6000 or 150 mM NaCl. Subcellular localization showed that the gene was localized in the nucleus. The germination rate and root length of the ZmSRG7 overexpressing lines were significantly increased under drought or salt stress compared with the control. However, after drought stress, the survival rate and relative water content of maize were increased, while the water loss rate was slowed down. Under salt stress, the Na+ concentration and Na+: K+ ratio of maize was increased. In addition, the contents of antioxidant enzymes and proline in maize under drought or salt stress were higher than those in the control, while the contents of MDA, H2O2 and O2- were lower than those in the control. The results showed that the ZmSRG7 gene played its biological function by regulating the ROS signaling pathway. An interaction between ZmSRG7 and the Zmdhn1 protein was found using a yeast two-hybrid experiment. These results suggest that the ZmSRG7 gene can improve maize tolerance to drought or salt by regulating hydrogen peroxide homeostasis.

UI MeSH Term Description Entries
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant
D003313 Zea mays A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER. Corn,Indian Corn,Maize,Teosinte,Zea,Corn, Indian
D006861 Hydrogen Peroxide A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials. Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D000040 Abscisic Acid Abscission-accelerating plant growth substance isolated from young cotton fruit, leaves of sycamore, birch, and other plants, and from potatoes, lemons, avocados, and other fruits. 2,4-Pentadienoic acid, 5-(1-hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexen-1-yl)-3-methyl-, (S-(Z,E))-,Abscisic Acid Monoammonium Salt, (R)-Isomer,Abscisic Acid, (+,-)-Isomer,Abscisic Acid, (E,E)-(+-)-Isomer,Abscisic Acid, (E,Z)-(+,-)-Isomer,Abscisic Acid, (R)-Isomer,Abscisic Acid, (Z,E)-Isomer,Abscissic Acid,Abscissins
D013312 Stress, Physiological The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions. Biotic Stress,Metabolic Stress,Physiological Stress,Abiotic Stress,Abiotic Stress Reaction,Abiotic Stress Response,Biological Stress,Metabolic Stress Response,Physiological Stress Reaction,Physiological Stress Reactivity,Physiological Stress Response,Abiotic Stress Reactions,Abiotic Stress Responses,Abiotic Stresses,Biological Stresses,Biotic Stresses,Metabolic Stress Responses,Metabolic Stresses,Physiological Stress Reactions,Physiological Stress Responses,Physiological Stresses,Reaction, Abiotic Stress,Reactions, Abiotic Stress,Response, Abiotic Stress,Response, Metabolic Stress,Stress Reaction, Physiological,Stress Response, Metabolic,Stress Response, Physiological,Stress, Abiotic,Stress, Biological,Stress, Biotic,Stress, Metabolic
D014867 Water A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Hydrogen Oxide
D055049 Salt Tolerance The ability of organisms to sense and adapt to high concentrations of salt in their growth environment. Salt-Tolerance,Saline-Tolerance,Salinity Tolerance,Salt Adaptation,Salt Adaption,Salt-Adaption,Adaptation, Salt,Adaption, Salt,Saline Tolerance,Salinity Tolerances,Salt Adaptations,Salt Adaptions,Salt Tolerances,Tolerance, Salinity,Tolerance, Salt
D055864 Droughts Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors. Drought
D018506 Gene Expression Regulation, Plant Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D030821 Plants, Genetically Modified PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING. Genetically Modified Plants,Plants, Transgenic,Transgenic Plants,GMO Plants,Genetically Engineered Plants,Engineered Plant, Genetically,Engineered Plants, Genetically,GMO Plant,Genetically Engineered Plant,Genetically Modified Plant,Modified Plant, Genetically,Modified Plants, Genetically,Plant, GMO,Plant, Genetically Engineered,Plant, Genetically Modified,Plant, Transgenic,Plants, GMO,Plants, Genetically Engineered,Transgenic Plant

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